Acetylation of the CspA family protein CspC controls the type III secretion system through translational regulation ofexsAinPseudomonas aeruginosa
| Autor: | Xuetao Gong, Weihui Wu, Zhuo Yue, Xiaolei Pan, Zhihui Cheng, Yuding Weng, Yongxin Jin, Fang Bai, Xinxin Zhang, Shouyi Li, Xiaoxiao Li, Zhouyi Chai |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
AcademicSubjects/SCI00010
Virulence Biology medicine.disease_cause Type three secretion system Mice 03 medical and health sciences Bacterial Proteins Gene expression Translational regulation Pneumonia Bacterial Type III Secretion Systems Genetics medicine Animals Humans Promoter Regions Genetic Gene Heat-Shock Proteins 030304 developmental biology Regulation of gene expression 0303 health sciences 030306 microbiology Pseudomonas aeruginosa Gene regulation Chromatin and Epigenetics RNA Acetylation Gene Expression Regulation Bacterial Cell biology A549 Cells Protein Biosynthesis Trans-Activators |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkab506 |
| Popis: | The ability to fine tune global gene expression in response to host environment is critical for the virulence of pathogenic bacteria. The host temperature is exploited by the bacteria as a cue for triggering virulence gene expression. However, little is known about the mechanism employed by Pseudomonas aeruginosa to response to host body temperature. CspA family proteins are RNA chaperones that modulate gene expression. Here we explored the functions of P. aeruginosa CspA family proteins and found that CspC (PA0456) controls the bacterial virulence. Combining transcriptomic analyses, RNA-immunoprecipitation and high-throughput sequencing (RIP-Seq), we demonstrated that CspC represses the type III secretion system (T3SS) by binding to the 5′ untranslated region of the mRNA of exsA, which encodes the T3SS master regulatory protein. We further demonstrated that acetylation at K41 of the CspC reduces its affinity to nucleic acids. Shifting the culture temperature from 25°C to 37°C or infection of mouse lung increased the CspC acetylation, which derepressed the expression of the T3SS genes, resulting in elevated virulence. Overall, our results identified the regulatory targets of CspC and revealed a regulatory mechanism of the T3SS in response to temperature shift and host in vivo environment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|