Implication of Soluble HLA-G and HLA-G +3142G/C Polymorphism in Breast Cancer Patients Receiving Adjuvant Therapy in Tanzania
| Autor: | Lucy Namkinga, Nazima Dharsee, Teddy Mselle, Ismael Adolf, Gokce Akan, Fatmahan Atalar |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Oncology Adult medicine.medical_specialty Genotype Breast cancer diagnosis medicine.medical_treatment Tanzanian population Breast Neoplasms Human leukocyte antigen HLA-C Antigens Malignancy Tanzania 03 medical and health sciences 0302 clinical medicine Breast cancer Gene Frequency HLA-G Internal medicine Adjuvant therapy Tumor Microenvironment Medicine SNP Humans Genetic Predisposition to Disease Genotyping Alleles HLA-G Antigens Polymorphism Genetic business.industry mastectomy General Medicine Middle Aged medicine.disease Prognosis Combined Modality Therapy soluble HLA-G 030104 developmental biology HLA-G +3142G/C 030220 oncology & carcinogenesis Case-Control Studies Disease Progression Female business Mastectomy Research Article |
| Zdroj: | Asian Pacific Journal of Cancer Prevention : APJCP |
| ISSN: | 2476-762X |
| Popis: | Background: During cancer growth, immunosuppressive microenvironment is created that enables tumour cells to evade an eliminative immune response and hence manage to grow into malignancy. HLA-G, existing as either membrane-bound (mHLA-G) or soluble (sHLA-G) molecule is thought to be immunosuppressive and produced more by tumor cells. The +3142G/C polymorphism in HLA-G gene affects its expression, and G allele is considered to be a protective mutant allele associated with less expression of HLA-G. The implication of HLA-G in cancer development has been reported in different cancers and populations. But, its implication in most African populations has not yet been investigated. The aim of this study was to determine the possible associations of soluble HLA-G and HLA-G +3142G/C SNP with breast cancer. Materials and Methods: 75 breast cancer patients and 84 normal controls were recruited in this study. The genotyping of HLA-G +3142G/C polymorphism was determined by LightSNiP typing assay using quantitative Real-Time PCR and sHLA-G levels were determined by ELISA. Results: The sHLA-G levels were significantly lower in breast cancer patients than in controls (p0.05) and was neither associated with sHLA-G levels. Conclusion: While the +3142G/C SNP was found not to be relevant to breast cancer, the changes of sHLA-G levels in response to medical interventions such as mastectomy may be translated into its potential prognostic utility for breast cancer. More studies are needed to provide clear evidence of sHLA-G as a diagnostic and prognostic marker of breast cancer in Tanzania. |
| Databáze: | OpenAIRE |
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