Exercise restores decreased physical activity levels and increases markers of autophagy and oxidative capacity in myostatin/activin blocked mdx mice
| Autor: | Willem M.H. Hoogaars, Olli Ritvos, Mika Silvennoinen, Bernardo Moreira Soares Oliveira, Heikki Kainulainen, Arja Pasternack, Juha J. Hulmi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
medicine.medical_specialty
Physiology Activin Receptors Type II Endocrinology Diabetes and Metabolism Blotting Western Citrate (si)-Synthase Myostatin Motor Activity Hematocrit Muscle hypertrophy Eating Hemoglobins Mice 03 medical and health sciences 0302 clinical medicine Physical Conditioning Animal Physiology (medical) Internal medicine Autophagy medicine Animals Muscle Skeletal ta315 Creatine Kinase Adiposity 030304 developmental biology 0303 health sciences biology medicine.diagnostic_test Tumor Necrosis Factor-alpha Body Weight Skeletal muscle DNA Activin receptor Activins Mice Inbred C57BL medicine.anatomical_structure Endocrinology Mice Inbred mdx biology.protein Creatine kinase Tumor necrosis factor alpha Oxidation-Reduction 030217 neurology & neurosurgery |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 305(2):E171-E182 |
| ISSN: | 0193-1849 |
| DOI: | 10.1152/ajpendo.00065.2013 |
| Popis: | The importance of adequate levels of muscle size and function and physical activity is widely recognized. Myostatin/activin blocking increases skeletal muscle mass but may decrease muscle oxidative capacity and can thus be hypothesized to affect voluntary physical activity. Soluble activin receptor IIB (sActRIIB-Fc) was produced to block myostatin/activins. Modestly dystrophic mdx mice were injected with sActRIIB-Fc or PBS with or without voluntary wheel running exercise for 7 wk. Healthy mice served as controls. Running for 7 wk attenuated the sActRIIB-Fc-induced increase in body mass by decreasing fat mass. Running also enhanced/restored the markers of muscle oxidative capacity and autophagy in mdx mice to or above the levels of healthy mice. Voluntary running activity was decreased by sActRIIB-Fc during the first 3–4 wk correlating with increased body mass. Home cage physical activity of mice, quantified from the force plate signal, was decreased by sActRIIB-Fc the whole 7-wk treatment in sedentary mice. To understand what happens during the first weeks after sActRIIB-Fc administration, when mice are less active, healthy mice were injected with sActRIIB-Fc or PBS for 2 wk. During the sActRIIB-Fc-induced rapid 2-wk muscle growth period, oxidative capacity and autophagy were reduced, which may possibly explain the decreased running activity. These results show that increased muscle size and decreased markers of oxidative capacity and autophagy during the first weeks of myostatin/activin blocking are associated with decreased voluntary activity levels. Voluntary exercise in dystrophic mice enhances the markers of oxidative capacity and autophagy to or above the levels of healthy mice. |
| Databáze: | OpenAIRE |
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