Diagnosis of pulmonary histoplasmosis and blastomycosis by detection of antigen in bronchoalveolar lavage fluid using an improved second-generation enzyme-linked immunoassay
| Autor: | Michelle Durkin, Michelle Parker, Lindsey Egan, L. Joseph Wheat, Kenneth S. Knox, DeAnna Fuller, Deborah Blue-Hnidy, Ann M. LeMonte, Chadi A. Hage, Patricia Connelly, Thomas E. Davis |
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| Rok vydání: | 2007 |
| Předmět: |
Bronchoalveolar lavage
Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Antigens Fungal medicine.medical_treatment Histoplasma Enzyme-Linked Immunosorbent Assay Liver transplantation Statistics Nonparametric Histoplasmosis Blastomycosis Second-generation assay Immunocompromised Host Antigen Humans Medicine Mycosis Chi-Square Distribution Lung Diseases Fungal medicine.diagnostic_test biology business.industry Respiratory disease Middle Aged medicine.disease biology.organism_classification Liver Transplantation Histoplasma antigen Immunology Female business Bronchoalveolar Lavage Fluid |
| Zdroj: | Respiratory Medicine. 101(1):43-47 |
| ISSN: | 0954-6111 |
| DOI: | 10.1016/j.rmed.2006.04.017 |
| Popis: | SummaryAntigen detection is a useful adjunct for the diagnosis of histoplasmosis. The purpose of this study was to evaluate antigen detection in bronchoalveolar lavage (BAL) fluid using an improved second-generation Histoplasma antigen assay. Antigen was detected in 16 of 19 (84%) cases of histoplasmosis and 5 of 6 (83.3%) blastomycosis cases using the second-generation assay vs. 13 of 19 (68%) and 4 of 6 (66.7%), respectively, in the original assay. Ten-fold concentration permitted detection of antigen in an additional case of histoplasmosis and another with blastomycosis, for an overall sensitivity of 23 of 25 (92.0%). Specificity was 98.2% in both assays in controls with other pulmonary infections. These findings support the diagnostic utility of the second-generation assay in patients with pulmonary histoplasmosis and blastomycosis. |
| Databáze: | OpenAIRE |
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