The Panel of 12 Cell-Free MicroRNAs as Potential Biomarkers in Prostate Neoplasms
Autor: | S. V. Yarmoschuk, Svetlana V. Pak, Olga E. Bryzgunova, Ivan A. Zaporozhchenko, O. A. Pashkovskaya, P. P. Laktionov, Maria Yu. Konoshenko, Evgeniy A. Lekchnov |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Oncology medicine.medical_specialty Clinical Biochemistry Urine Cell free Extracellular vesicles Article 03 medical and health sciences Prostate cancer 0302 clinical medicine Internal medicine microRNA medicine lcsh:R5-920 business.industry Hyperplasia medicine.disease prostate cancer urine 030104 developmental biology 030220 oncology & carcinogenesis Potential biomarkers Prostate neoplasm business extracellular vesicles lcsh:Medicine (General) mirna |
Zdroj: | Diagnostics, Vol 10, Iss 1, p 38 (2020) Diagnostics Volume 10 Issue 1 |
ISSN: | 2075-4418 |
Popis: | Prostate cancer is a global biological, medical, and social issue aggravated by the lack of reliable, highly specific, and sensitive non-invasive tests for diagnosis and staging of prostate cancer. One prospective source of biomarkers are the cell-free miRNAs present in various biological fluids. In the present study, we validated the diagnostic potential of cell-free miRNAs: miR-19b, miR-22, miR-92a, miR-378, miR-425, miR-30e, miR-31, miR-125b, miR-200b, miR-205, miR-375, and miR-660 we estimated the required sample size and the minimal miRNA set for a subsequent large-scale validation study. Relative expression of 12 miRNA combined in 31 ratios was investigated in three fractions of biological fluids (urine extracellular vesicles, clarified urine, and plasma) obtained from patients with prostate cancer (n = 10), benign prostate hyperplasia (n = 8), and healthy volunteers (n = 11). Eight of the miRNAs found in urine vesicles (miR-19b, miR-30e, miR-31, miR-92a, miR-125, miR-200, miR-205, and miR-660) showed great promise and when combined into six ratios (miR-125b/miR-30e, miR-200/miR-30e, miR-205/miR-30e, miR-31/miR-30e, miR-660/miR-30e, and miR-19b/miR-92a) could classify patients with prostate cancer, benign prostate hyperplasia, and healthy donors with 100% specificity, 100% sensitivity, and with a high degree of reliability for most donors. |
Databáze: | OpenAIRE |
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