Confirmed case of encephalitis caused by Murray Valley encephalitis virus infection in a horse
| Autor: | Anita N. Gordon, John Bingham, David T. Williams, Glen Edmunds, Cameron R. Marbach, Jane Oakey, Sinead M. Diviney, Kelly Condon |
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| Rok vydání: | 2012 |
| Předmět: |
Pathology
medicine.medical_specialty Encephalomyelitis Molecular Sequence Data Encephalitis Virus Murray Valley Biology medicine.disease_cause Murray Valley encephalitis virus Virus Fatal Outcome Viral Envelope Proteins Veterinary virology medicine Animals Horses Phylogeny Base Sequence General Veterinary Reverse Transcriptase Polymerase Chain Reaction Encephalitis Arbovirus Sequence Analysis DNA medicine.disease Spinal cord biology.organism_classification Immunohistochemistry Virology Reverse transcription polymerase chain reaction Flavivirus medicine.anatomical_structure RNA Viral Horse Diseases Queensland Sequence Alignment Encephalitis |
| Zdroj: | Journal of Veterinary Diagnostic Investigation. 24:431-436 |
| ISSN: | 1943-4936 1040-6387 |
| Popis: | A 5-year-old Australian stock horse in Monto, Queensland, Australia, developed neurological signs and was euthanized after a 6-day course of illness. Histological examination of the brain and spinal cord revealed moderate to severe subacute, nonsuppurative encephalomyelitis. Sections of spinal cord stained positively in immunohistochemistry with a flavivirus-specific monoclonal antibody. Reverse transcription polymerase chain reaction assay targeting the envelope gene of flavivirus yielded positive results from brain, spinal cord, cerebrospinal fluid, and facial nerve. A flavivirus was isolated from the cerebrum and spinal cord. Nucleotide sequences obtained from amplicons from both tissues and virus isolated in cell culture were compared with those in GenBank and had 96–98% identity with Murray Valley encephalitis virus. The partial envelope gene sequence of the viral isolate clustered into genotype 1 and was most closely related to a previous Queensland isolate. |
| Databáze: | OpenAIRE |
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