Spider venoms inhibit L-glutamate binding to brain synaptic membrane receptors
| Autor: | N Galton, S L Early, E K Michaelis |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Male
Spider Venoms Synaptic Membranes Glutamic Acid Venom Receptors Cell Surface Biology Inhibitory postsynaptic potential complex mixtures Araneus gemma Glutamates Species Specificity Animals Receptor Arthropod Venoms Multidisciplinary Glutamate receptor Brain Rats Inbred Strains Glutamic acid biology.organism_classification Rats Kinetics Biochemistry Receptors Glutamate Argiope aurantia Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 81(17) |
| ISSN: | 0027-8424 |
| Popis: | The venoms from three spider species, Araneus gemma, Neoscona arabesca, and Argiope aurantia, were shown to inhibit the high-affinity, sodium-independent L-glutamate-binding sites in rat brain synaptic membranes. The same three venoms caused concentration-dependent inhibition of the activity of the glutamate-binding glycoprotein purified from rat brain synaptic membranes. The venom milked from the glands of Araneus gemma was the most active inhibitor of L-glutamate binding, causing 60-80% inhibition of both synaptic membrane and purified protein binding activity at 0.01 unit. The inhibitory activity of this venom was associated with a single protein peak obtained from gel permeation chromatography of the venom. Finally, the effect of the venom from Araneus gemma on the synaptic membrane glutamate-binding sites was slowly reversible. These observations indicate that the spider venoms have a direct effect on the recognition sites for L-glutamic acid in brain synaptic membranes and that these sites are related to the physiologic glutamate receptors. |
| Databáze: | OpenAIRE |
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