Cryo-EM structure of ssDNA bacteriophage ΦCjT23 provides insight into early virus evolution
| Autor: | Nejc Kejzar, Elina Laanto, Ilona Rissanen, Vahid Abrishami, Muniyandi Selvaraj, Sylvain Moineau, Janne Ravantti, Lotta-Riina Sundberg, Juha T. Huiskonen |
|---|---|
| Přispěvatelé: | Institute of Biotechnology (-2009), Helsinki Institute of Life Science HiLIFE, Joint Activities, Molecular and Integrative Biosciences Research Programme, Molecular Principles of Viruses, Institute of Biotechnology, Helsinki Institute of Life Science HiLIFE, Biosciences, Laanto, Elina [0000-0003-4172-3128], Rissanen, Ilona [0000-0003-4937-1825], Abrishami, Vahid [0000-0002-3772-7663], Selvaraj, Muniyandi [0000-0001-7793-6323], Moineau, Sylvain [0000-0002-2832-5101], Sundberg, Lotta-Riina [0000-0003-3510-4398], Huiskonen, Juha T [0000-0002-0348-7323], Apollo - University of Cambridge Repository |
| Rok vydání: | 2022 |
| Předmět: |
631/326/1321
bacteriophages virukset cryoelectron microscopy evoluutio General Physics and Astronomy elektronimikroskopia DNA Single-Stranded 45/23 Flavobacterium General Biochemistry Genetics and Molecular Biology bakteriofagit 631/45/535/1258/1259 viral evolution 631/326/596/2554 Bacteriophages Multidisciplinary fylogenia 45 fylogenetiikka Cryoelectron Microscopy 101/28 article General Chemistry perimä 1182 Biochemistry cell and molecular biology Capsid Proteins |
| DOI: | 10.17863/cam.91430 |
| Popis: | The origin of viruses remains an open question. While lack of detectable sequence similarity hampers the analysis of distantly related viruses, structural biology investigations of conserved capsid protein structures facilitate the study of distant evolutionary relationships. Here we characterize the lipid-containing ssDNA temperate bacteriophage ΦCjT23, which infects Flavobacterium sp. (Bacteroidetes). We report ΦCjT23-like sequences in the genome of strains belonging to several Flavobacterium species. The virion structure determined by cryogenic electron microscopy reveals similarities to members of the viral kingdom Bamfordvirae that currently consists solely of dsDNA viruses with a major capsid protein composed of two upright β-sandwiches. The minimalistic structure of ΦCjT23 suggests that this phage serves as a model for the last common ancestor between ssDNA and dsDNA viruses in the Bamfordvirae. Both ΦCjT23 and the related phage FLiP infect Flavobacterium species found in several environments, suggesting that these types of viruses have a global distribution and a shared evolutionary origin. Detailed comparisons to related, more complex viruses not only expand our knowledge about this group of viruses but also provide a rare glimpse into early virus evolution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|