Slr1670 from Synechocystis sp. PCC 6803 Is Required for the Re-assimilation of the Osmolyte Glucosylglycerol
| Autor: | Xuefeng Lu, Cuncun Qiao, Klaas J. Hellingwerf, Kuo Song, Philipp Savakis, Xiaoming Tan, Filipe Branco dos Santos |
|---|---|
| Přispěvatelé: | Molecular Microbial Physiology (SILS, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cyanobacteria Microbiology (medical) Glycerol kinase osmolyte 030106 microbiology lcsh:QR1-502 Biology Microbiology cyanobacteria lcsh:Microbiology Slr1670 03 medical and health sciences chemistry.chemical_compound Glycerol Glycoside hydrolase Original Research salt stress glucosylglycerol Synechocystis biology.organism_classification De novo synthesis 030104 developmental biology Biochemistry chemistry Osmolyte Archaea |
| Zdroj: | Frontiers in Microbiology, Vol 7 (2016) Frontiers in Microbiology, 7:1350. Frontiers Media S.A. Frontiers in Microbiology |
| ISSN: | 1664-302X |
| Popis: | When subjected to mild salt stress, the cyanobacterium Synechocystis sp. PCC 6803 produces small amounts of glycerol through an as of yet unidentified pathway. Here, we show that this glycerol is a degradation product of the main osmolyte of this organism, glucosylglycerol (GG). Inactivation of ggpS, encoding the first step of GG-synthesis, abolished de novo synthesis of glycerol, while the ability to hydrolyze exogenously supplied glucoslylglycerol was unimpaired. Inactivation of glpK, encoding glycerol kinase, had no effect on glycerol synthesis. Inactivation of slr1670, encoding a GHL5-type putative glycoside hydrolase, abolished de novo synthesis of glycerol, as well as hydrolysis of GG, and led to increased intracellular concentrations of this osmolyte. Slr1670 therefore presumably displays GG hydrolase activity. A gene homologous to the one encoded by slr1670 occurs in a wide range of cyanobacteria, proteobacteria, and archaea. In cyanobacteria, it co-occurs with genes involved in GG-synthesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|