The proteome of the presynaptic active zone from mouse brain
| Autor: | Melanie Laßek, Jens Weingarten, Marion Rohmer, Ilaria Lunger, Simone Dudek, Walter Volknandt, Benjamin F. Mueller, Michael Karas, Patricia Gogesch, Dominic Baeumlisberger |
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| Rok vydání: | 2014 |
| Předmět: |
Proteome
Mutant Presynaptic Terminals Synaptic Membranes Synaptic vesicle Mice Cellular and Molecular Neuroscience chemistry.chemical_compound Western blot medicine Animals Active zone Neurotransmitter Molecular Biology Glyceraldehyde 3-phosphate dehydrogenase biology medicine.diagnostic_test Brain Cell Biology Cell biology Mice Inbred C57BL chemistry biology.protein Synaptic Vesicles Presynaptic active zone |
| Zdroj: | Molecular and Cellular Neuroscience. 59:106-118 |
| ISSN: | 1044-7431 |
| DOI: | 10.1016/j.mcn.2014.02.003 |
| Popis: | Neurotransmitter release as well as the structural and functional dynamics of the presynaptic active zone is controlled by proteinaceous components. Here we describe for the first time an experimental approach for the isolation of the presynaptic active zone from individual mouse brains, a prerequisite for understanding the functional inventory of the presynaptic protein network and for the later analysis of changes occurring in mutant mice. Using a monoclonal antibody against the ubiquitous synaptic vesicle protein SV2 we immunopurified synaptic vesicles docked to the presynaptic plasma membrane. Enrichment studies by means of Western blot analysis and mass spectrometry identified 485 proteins belonging to an impressive variety of functional categories. Our data suggest that presynaptic active zones represent focal hot spots that are not only involved in the regulation of neurotransmitter release but also in multiple structural and functional alterations the adult nerve terminal undergoes during neural activity in adult CNS. They furthermore open new avenues for characterizing alterations in the active zone proteome of mutant mice and their corresponding controls, including the various mouse models of neurological diseases. |
| Databáze: | OpenAIRE |
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