PARP1- and CTCF-Mediated Interactions between Active and Repressed Chromatin at the Lamina Promote Oscillating Transcription
| Autor: | Xingqi Chen, Marta P. Imreh, Olga Loseva, Anna Lewandowska Ronnegren, Thomas Helleday, Lluís Millan-Ariño, J. Peter Svensson, Samer Yammine, Noriyuki Sumida, Maria Israelsson, Emmanouil G. Sifakis, Balázs Németi, Anita Göndör, Farzaneh Shahin Varnoosfaderani, Barbara A. Scholz, Erik Fredlund, Honglei Zhao, Chengxi Shi, Carolina Diettrich Mallet de Lima, Li Sophie Zhao Rathje |
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| Rok vydání: | 2015 |
| Předmět: |
CCCTC-Binding Factor
Chromatin Immunoprecipitation Transcription Genetic Human Embryonic Stem Cells Poly (ADP-Ribose) Polymerase-1 Cell Cycle Proteins Biology Humans Gene Regulatory Networks Circadian rhythm Transcriptional attenuation Molecular Biology Embryoid Bodies Chromatin Fiber Adaptor Proteins Signal Transducing Regulation of gene expression Genetics Nuclear Lamina Membrane Proteins Epistasis Genetic Cell Biology HCT116 Cells Chromatin Cell biology Circadian Rhythm Repressor Proteins Gene Expression Regulation CTCF Nuclear lamina RNA Long Noncoding Poly(ADP-ribose) Polymerases Chromatin immunoprecipitation Protein Binding |
| Zdroj: | Molecular Cell. 59(6):984-997 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2015.07.019 |
| Popis: | Transcriptionally active and inactive chromatin domains tend to segregate into separate sub-nuclear compartments to maintain stable expression patterns. However, here we uncovered an inter-chromosomal network connecting active loci enriched in circadian genes to repressed lamina-associated domains (LADs). The interactome is regulated by PARP1 and its co-factor CTCF. They not only mediate chromatin fiber interactions but also promote the recruitment of circadian genes to the lamina. Synchronization of the circadian rhythm by serum shock induces oscillations in PARP1-CTCF interactions, which is accompanied by oscillating recruitment of circadian loci to the lamina, followed by the acquisition of repressive H3K9me2 marks and transcriptional attenuation. Furthermore, depletion of H3K9me2/3, inhibition of PARP activity by olaparib, or downregulation of PARP1 or CTCF expression counteracts both recruitment to the envelope and circadian transcription. PARP1- and CTCF-regulated contacts between circadian loci and the repressive chromatin environment at the lamina therefore mediate circadian transcriptional plasticity. |
| Databáze: | OpenAIRE |
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