| Autor: |
Eva Grafahrend-Belau, Nese Sreenivasulu, Hardy Rolletschek, Peter M. Jakob, Falk Schreiber, Thomas Altmann, Diana Weier, Twan Rutten, Nicolas Heinzel, Ljudmilla Borisjuk, Gerd Melkus, Volodymyr Radchuk, Johannes Fuchs |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Plant Biotechnology Journal. 9:1022-1037 |
| ISSN: |
1467-7644 |
| DOI: |
10.1111/j.1467-7652.2011.00618.x |
| Popis: |
Summary Seed growth and accumulation of storage products relies on the delivery of sucrose from the maternal to the filial tissues. The transport route is hidden inside the seed and has never been visualized in vivo. Our approach, based on high-field nuclear magnetic resonance and a cus- tom made 13 C ⁄ 1 H double resonant coil, allows the non-invasive imaging and monitoring of sucrose allocation within the seed. The new technique visualizes the main stream of sucrose and determines its velocity during the grain filling in barley (Hordeum vulgare L.). Quantifiable dynamic images are provided, which allow observing movement of 13 C-sucrose at a sub-milli- metre level of resolution. The analysis of genetically modified barley grains (Jekyll transgenic lines, seg8 and Riso13 mutants) demonstrated that sucrose release via the nucellar projection towards the endosperm provides an essential mean for the control of seed growth by mater- nal organism. The sucrose allocation was further determined by structural and metabolic fea- tures of endosperm. Sucrose monitoring was integrated with an in silico flux balance analysis, representing a powerful platform for non-invasive study of seed filling in crops. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
|
Nepřihlášeným uživatelům se plný text nezobrazuje |
K zobrazení výsledku je třeba se přihlásit.
|
