Comparative impact of cephaloridine on glutathione and related enzymes in LLC-PK1, LLC-RK1, and primary cultures of rat and rabbit proximal tubule cells
| Autor: | Christelle Monteil, Jean-Paul Morin, S. Marouillat, C. Lendormi |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Pharmacotoxicology
Swine Health Toxicology and Mutagenesis Biology Toxicology Kidney Tubules Proximal chemistry.chemical_compound Species Specificity medicine Cephaloridine Animals Rats Wistar Cells Cultured Glutathione Transferase Kidney urogenital system Cell Biology Glutathione Molecular biology Epithelium In vitro Enzymes Rats medicine.anatomical_structure Biochemistry chemistry Cell culture LLC-PK1 Cells Alkaline phosphatase Female Rabbits medicine.drug |
| Zdroj: | Cell Biology and Toxicology. 12:275-282 |
| ISSN: | 1573-6822 0742-2091 |
| DOI: | 10.1007/bf00438158 |
| Popis: | Among kidney tubular epithelial cell types, proximal tubule cells are one of the major renal targets for xenobiotics. Several in vitro culture models have been proposed for use of proximal tubule cells for in vitro pharmacotoxicology studies. This paper reports a comparative study of the response to cephaloridine exposure of two established cell lines from pig (LLC-PK1) and rabbit (LLC-RK1) kidneys and primary cultures of rat and rabbit proximal tubule cells. These cultured cells were first compared for their levels of activity of alpha-methylglucopyranoside transport, alkaline phosphatase, succinate dehydrogenase, and NADPH cytochrome c reductase, their glutathione-dependent activity levels, and their adenylate cyclase response pattern to stimulation by PTH and AVP. The results presented show major phenotypic differences between these four cellular models. The differences observed in glutathione-dependent mechanism activities and regulation may in part be responsible for the variability of the responses of these four cellular models when exposed to cephaloridine. |
| Databáze: | OpenAIRE |
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