WISP-3 inhibition of miR-452 promotes VEGF-A expression in chondrosarcoma cells and induces endothelial progenitor cells angiogenesis

Autor: Yi Lee, Wei Hung Yang, Yi Chen Yang, Chih-Hsin Tang, Chih-Yang Lin, Hsien-Te Chen, Te-Mao Li, Huey En Tzeng, Shih-Wei Wang
Rok vydání: 2017
Předmět:
Vascular Endothelial Growth Factor A
0301 basic medicine
Angiogenesis
Chondrosarcoma
Bone Neoplasms
p38 Mitogen-Activated Protein Kinases
VEGF-A
miR-452
Metastasis
CCN Intercellular Signaling Proteins
CSK Tyrosine-Protein Kinase
Mice
angiogenesis
03 medical and health sciences
0302 clinical medicine
Cell Movement
Cell Line
Tumor
medicine
Animals
Humans
Progenitor cell
Cell Proliferation
Endothelial Progenitor Cells
Neovascularization
Pathologic
Traditional medicine
business.industry
WISP-3
medicine.disease
Gene Expression Regulation
Neoplastic
Disease Models
Animal
MicroRNAs
Vascular endothelial growth factor A
src-Family Kinases
030104 developmental biology
Oncology
Gene Knockdown Techniques
030220 oncology & carcinogenesis
Cancer research
Heterografts
Osteosarcoma
Signal transduction
business
Signal Transduction
Research Paper
Zdroj: Oncotarget
ISSN: 1949-2553
DOI: 10.18632/oncotarget.17142
Popis: Chondrosarcoma is the second most prevalent general primary tumor of bone following osteosarcoma. Chondrosarcoma development may be linked to angiogenesis, which is principally elicited by vascular endothelial growth factor-A (VEGF-A). VEGF-A level has been recognized as a prognostic marker in angiogenesis. WNT1-inducible signaling pathway protein-3 (WISP)-3/CCN6 belongs to the CCN family and is involved in regulating several cellular functions, including cell proliferation, differentiation, and migration. Nevertheless, the effect of WISP-3 on VEGF-A production and angiogenesis in human chondrosarcoma remains largely unknown. This current study shows that WISP-3 promoted VEGF-A production and induced angiogenesis of human endothelial progenitor cells. Moreover, WISP-3-enhanced VEGF-A expression and angiogenesis involved the c-Src and p38 signaling pathways, while miR-452 expression was negatively affected by WISP-3 via the c-Src and p38 pathways. Our results illustrate the clinical significance of WISP-3, VEGF-A and miR-452 in human chondrosarcoma patients. WISP-3 may illustrate a novel therapeutic target in the metastasis and angiogenesis of chondrosarcoma.
Databáze: OpenAIRE
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