In vitro assessment of liposomal neridronate on MDA-MB-231 human breast cancer cells
| Autor: | Imène Chebbi, Odile Sainte-Catherine, Olivier Seksek, Marc Lecouvey, Evelyne Migianu-Griffoni |
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| Přispěvatelé: | Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2010 |
| Předmět: |
MECHANISM
Pathology medicine.medical_specialty INHIBITS ANGIOGENESIS Cell Survival media_common.quotation_subject Pharmaceutical Science Breast Neoplasms Capsules VIVO Flow cytometry ACTIVATION PATHWAY 03 medical and health sciences 0302 clinical medicine BISPHOSPHONATES Cell Line Tumor DELIVERY-SYSTEMS medicine Humans Human breast cancer cells Viability assay Internalization Cell Proliferation 030304 developmental biology media_common 0303 health sciences Liposome Diphosphonates medicine.diagnostic_test business.industry PROLIFERATION Cancer Cell migration medicine.disease Matrix Metalloproteinases In vitro 3. Good health Neridronate 030220 oncology & carcinogenesis ACID Liposomes Cancer cell Cancer research GROWTH Female Caco-2 Cells business |
| Zdroj: | International Journal of Pharmaceutics International Journal of Pharmaceutics, Elsevier, 2010, 383 (1-2), pp.116-122. ⟨10.1016/j.ijpharm.2009.09.011⟩ |
| ISSN: | 0378-5173 |
| Popis: | 1 - Article; Bisphosphonates have been used for decades in the standard therapy of bone-related diseases, including bone metastasis of various malignancies, and they might as well be toxic on early cancer cells themselves. In order to allow a better delivery of neridronate (a N-containing bisphosphonate with relatively poor activity), liposomes were evaluated in vitro on cancer cell lines (MDA-MB 231, U87-MG and Caco2). After chemical synthesis, this water-soluble molecule was encapsulated into liposomes containing DOPC:DOPG:Chol (72:27:1 molar ratio). The influence of neridronate (free or liposomal) on cell viability or proliferation after treatment was evaluated using the MTT method, as well as cell migration and invasion assays; these techniques showed a drastic improvement of the action of neridronate on MDA-MB-231 cells with an EC50 fifty times lower when neridronate was encapsulated. Internalization of liposomes was followed by flow cytometry and fluorescence microscopy, demonstrating internalization via the endocytic pathway. Furthermore, since over-expression of matrix metalloproteinases (particularly MMP-2 and MMP-9) has been correlated to poor prognosis in many cancer types, detection of MMP expression is a satisfactory indication of the therapy efficiency and was then performed on treated cells. On MDA-MB-231 cells, MPPs expression was also significantly reduced by neridronate while entrapped in liposomes. |
| Databáze: | OpenAIRE |
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