MiR-30a-5p promotes cholangiocarcinoma cell proliferation through targeting SOCS3
| Autor: | Xing Wang, Wang Jie Jiang, Dong Wang, Yao Dong Zhang, Gao Chao Li, Xiangcheng Li, Chang Xian Li, Chen Huan Luo, Hong Wei Wang, Jia Wei Zhang, Sheng Han |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Reporter gene Cell growth proliferation miR-30a-5p apoptosis Biology In vitro 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology Apoptosis 030220 oncology & carcinogenesis microRNA Cancer research Luciferase Clinical significance SOCS3 cholangiocarcinoma Research Paper |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| DOI: | 10.7150/jca.41437 |
| Popis: | Background: MicroRNAs (miRNAs) play important roles in the occurrence and development of cancers. In this project, we aimed to explore the role and molecular mechanism of mir-30a-5p in cholangiocarcinoma (CCA). Materials and Methods: The expression profile and clinical significance of miR-30a-5p in CCA patients were investigated in 31 ICC and 52 ECC patients respectively. The role and mechanism of miR-30a-5p in CCA cells were investigated by up-regulating and inhibiting miR-30a-5p expression in vitro functional study. Results: The expression of miR-30a-5p was increased in both CCA tissues and cells. The inhibition of miR-30a-5p decreased cell proliferation and induced cell apoptosis while overexpression of miR-30a-5p achieved the opposite effect. Furthermore, SOCS3 was down-regulated in ICC and ECC tissues and negatively regulated by miR-30a-5p. Dual-luciferase reporter assay revealed that co-transfection of miR-30a-5p significantly inhibited the activity of firefly luciferase reporter carrying the wild-type 3′UTR of SOCS3. The inhibition of SOCS3 could largely rescue the inhibitory effect of miR-30a-5p inhibition on CCA cells proliferation. In clinical, up-regulated miR-30a-5p expression was correlated with large tumor size in both ICC and ECC cohorts. Conclusions: miR-30a-5p promoted CCA cells proliferation through targeting SOCS3. These findings suggested that miR-30a-5p could be a potential therapeutic target. |
| Databáze: | OpenAIRE |
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