Neurotoxicity Screening in a Multipotent Neural Stem Cell Line Established from the Mouse Brain
| Autor: | Myeong-Kyu Kim, Yeoung-Geol Park, Hyung-Seok Kim, Hyun-Beom Choi, Han-Seong Jeong, Yong-Soo Choi, Jae-Kyu Ryu, Kyung-Hwa Lee, Young-Jong Woo, Seung-Up Kim, Hyun-Kyung Kim, Min-Cheol Lee |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Programmed cell death
Culture Neurotoxins Glutamic Acid Biology Cell Line Nestin Mice Growth Factors medicine Excitatory Amino Acid Agonists Cytotoxic T cell Animals Humans Cell Lineage Enzyme Inhibitors Buthionine Sulfoximine Neurons Mice Inbred BALB C Kainic Acid Stem Cells Multipotent Stem Cells Neurodegeneration Glutamate receptor Neurotoxicity Brain Cell Differentiation General Medicine Hydrogen Peroxide medicine.disease Oxidants Neural stem cell Cell biology Neuroepithelial cell Phenotype Biochemistry nervous system Cytokines Intercellular Signaling Peptides and Proteins Original Article Stem cell Reactive Oxygen Species Neuroglia Neuroscience |
| Zdroj: | Journal of Korean Medical Science |
| ISSN: | 1598-6357 1011-8934 |
| Popis: | Neural stem cells (NSCs) have mainly been applied to neurodegeneration in some medically intractable neurologic diseases. In this study, we established a novel NSC line and investigated the cytotoxic responses of NSCs to exogenous neurotoxicants, glutamates and reactive oxygen species (ROS). A multipotent NSC line, B2A1 cells, was established from long-term primary cultures of oligodendrocyte-enriched cells from an adult BALB/c mouse brain. B2A1 cells could be differentiated into neuronal, astrocytic and oligodendroglial lineages. The cells also expressed genotypic mRNA messages for both neural progenitor cells and differentiated neuronoglial cells. B2A1 cells treated with hydrogen peroxide and L-buthionine-(S,R)-sulfoximine underwent 30-40% cell death, while B2A1 cells treated with glutamate and kainate showed 25-35% cell death. Cytopathologic changes consisting of swollen cell bodies, loss of cytoplasmic processes, and nuclear chromatin disintegration, developed after exposure to both ROS and excitotoxic chemicals. These results suggest that B2A1 cells may be useful in the study of NSC biology and may constitute an effective neurotoxicity screening system for ROS and excitotoxic chemicals. |
| Databáze: | OpenAIRE |
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