Opposite Effects of the Spinach Food Matrix on Lutein Bioaccessibility and Intestinal Uptake Lead to Unchanged Bioavailability Compared to Pure Lutein

Autor: Catherine Caris-Veyrat, Pascale Goupy, Caroline Buffière, Patrick Borel, Charlotte Halimi, Marielle Margier, Emmanuelle Reboul, Didier Rémond
Přispěvatelé: Nutrition, obésité et risque thrombotique (NORT), Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research (C2VN), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre recherche en CardioVasculaire et Nutrition (C2VN), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU), Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Avignon Université (AU)-Institut National de la Recherche Agronomique (INRA)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Lutein
in vitro digestion
Swine
enterocyte
lutein isomer
chemistry.chemical_compound
Spinacia oleracea
Lipid droplet
Antineoplastic Combined Chemotherapy Protocols
Food science
Cooking
Carotenoid
Etoposide
chemistry.chemical_classification
Meal
biology
digestive
oral
and skin physiology
food and beverages
Postprandial Period
bioaccessibility
carotenoid
Postprandial
mini porc
minipigs
Swine
Miniature
Biotechnology
endocrine system
Biological Availability
03 medical and health sciences
lutéine
Animals
Humans
xanthophyll
Cyclophosphamide
intestine
lutein
030109 nutrition & dietetics
biology.organism_classification
Carotenoids
eye diseases
Bioavailability
030104 developmental biology
chemistry
Intestinal Absorption
Xanthophyll
Spinach
Prednisone
sense organs
Caco-2 Cells
Mitoxantrone
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
matrice alimentaire
Food Science
Zdroj: Molecular Nutrition and Food Research
Molecular Nutrition and Food Research, Wiley-VCH Verlag, 2018, 62 (11), ⟨10.1002/mnfr.201800185⟩
Molecular Nutrition and Food Research, 2018, 62 (11), ⟨10.1002/mnfr.201800185⟩
ISSN: 1613-4125
1613-4133
DOI: 10.1002/mnfr.201800185⟩
Popis: This work was supported by an INRA Priority Action of the "Alimentation Humaine" department (2013-2015). Marielle Margier received a doctoral fellowship (2015-2018) from Region PACA in partnership with the Centre Technique de Conservation des Produits Alimentaires (CTCPA, Avignon); Scope: Food matrix is generally believed to alter carotenoid bioavailability, but its effect on xanthophylls is usually limited. This study thus aims to decipher the digestion-absorption process of lutein in the presence or not of a food matrix.Methods: Lutein transfer to gastric-like lipid droplets or artificial mixed micelles was assessed when lutein was added to test meals either as a pure molecule ((all-E)-lutein) or in canned spinach ((Z) + (all-E)-lutein). The obtained mixed micelles were delivered to Caco-2 cells to evaluate lutein uptake. Finally postprandial plasma lutein responses were compared in minipigs after the two test meals.Results: Lutein transfer to gastric-like lipid droplets and to mixed micelles was higher when lutein was added in spinach than when it was added as pure lutein (+614% and +147%, respectively, p < 0.05). Conversely, lutein uptake was less effective when micellar lutein was from a meal containing spinach than from a meal containing its pure form (-55%, p < 0.05). In minipigs, postprandial lutein response was delayed with spinach but not significantly different after the two test meals.Conclusion: Opposite effects at the micellarization and intestinal cell uptake steps explain the lack of effect of spinach matrix on lutein bioavailability.
Databáze: OpenAIRE
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