Early rescue oocyte activation for activation-impaired oocytes with no second polar body extrusion after intracytoplasmic sperm injection
| Autor: | Nozomi Miyazaki, Hiroaki Kawato, Takashi Shibahara, Hiroyuki Minoura, Yuu Fukasaku, Naoko Hayashi |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male medicine.medical_treatment Embryonic Development Fertilization in Vitro Polar Bodies Intracytoplasmic sperm injection Andrology 03 medical and health sciences 0302 clinical medicine Human fertilization Genetics Medicine Humans Blastocyst Embryo Implantation Sperm Injections Intracytoplasmic Assisted Reproduction Technologies Genetics (clinical) reproductive and urinary physiology 030219 obstetrics & reproductive medicine business.industry urogenital system Embryogenesis Obstetrics and Gynecology Oocyte activation Second polar body General Medicine Oocyte Embryo Transfer Calcium Ionophores 030104 developmental biology medicine.anatomical_structure Reproductive Medicine embryonic structures Oocytes Female business Live Birth Developmental Biology |
| Zdroj: | J Assist Reprod Genet |
| ISSN: | 1573-7330 |
| Popis: | PURPOSE: When rescue artificial oocyte activation (ROA) is performed on the day after intracytoplasmic sperm injection (ICSI) or later, embryonic development is poor and seldom results in live births. The efficacy of an early ROA after ICSI is unclear. Is early ROA effective in rescuing unfertilized oocytes that have not undergone second polar body extrusion several hours after ICSI? METHODS: We performed retrospective cohort study between October 2016 and September 2019, targeting 2891 oocytes in 843 cycles when ICSI was performed. We performed ROA with calcium ionophore on 395 of the 475 oocytes with no second polar extrusion 2.5–6 h after ICSI. RESULTS: The normal fertilization rate of ROA oocytes was significantly higher than non-ROA oocytes (65.8% vs 6.7%, P < 0.001). The blastocyst development rate in ROA oocytes was significantly lower than spontaneously activated oocytes (48.9% vs 67.2%, P < 0.001). The ROA oocyte implantation rate did not significantly differ from the spontaneously activated oocytes (36.0% vs 41.2%). We observed no differences in the implantation rates and blastocyst development rates over the 2.5–6 h from ICSI until ROA. CONCLUSION: Early ROA is effective, and the optimal timing appears to be 2.5–6 h after ICSI. |
| Databáze: | OpenAIRE |
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