Induced Pluripotent Stem Cells: A New Approach for Physiological Research
| Autor: | Monireh Soroush Noghabi, Amir Morshedi, Markus Khalil, Jürgen Hescheler, Tobias Hannes, Kurt Pfannkuche, Peter Dröge |
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| Rok vydání: | 2010 |
| Předmět: |
Cell fusion
Physiology Somatic cell business.industry Induced Pluripotent Stem Cells Biology Cellular Reprogramming Embryonic stem cell Biotechnology Disease modelling Mice Cardiovascular Diseases Basic research Animals Humans Induced pluripotent stem cell business Cell potency Reprogramming Neuroscience Embryonic Stem Cells |
| Zdroj: | Cellular Physiology and Biochemistry. 26:105-124 |
| ISSN: | 1421-9778 1015-8987 |
| Popis: | The generation of induced pluripotent stem (iPS) cells by controlled delivery of reprogramming factors enables the derivation of pluripotent cells from a variety of somatic cell types. Patient-tailored iPS cells remove the major roadblock of immune rejection for clinical applications associated with the use of human embryonic stem (hES) cells. Beside therapeutic issues, iPS cell technology opens the door for broader research on human pluripotent cells because ethical limitations are lifted with iPS cells compared to hES cells. Scientists are now able to generate iPS cells for disease modelling and use them in basic research of physiological and pathophysiological models. In this concise review, we discuss the state of the art in the field of iPS cell induction by cell fusion or defined factors. Techniques to derive pluripotent cells from somatic sources are introduced and discussed, as well as some biological factors that influence the generation of iPS cells. We compare ES and iPS cells to answer the question whether these cells are identical, and we finish with an outlook on clinical research with iPS cells with a focus on cardiovascular medicine. |
| Databáze: | OpenAIRE |
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