Enhancement of Intestinal Immune Function in Mice by β-D-Glucan fromAureobasidium PullulansADK-34

Autor:	Shuichi Kaminogawa, K. Tsubaki, Satoshi Hachimura, H. Kawakami, K. Tanabe, A. Tanioka, Akira Hosono
Rok vydání:	2013
Předmět:	Male Immunoglobulin A beta-Glucans Immunology Microbiology Mice Immune system Adjuvants Immunologic Ascomycota Oral administration medicine Animals Secretion Cyclophosphamide Mice Inbred BALB C biology Interleukin General Medicine biology.organism_classification Small intestine ADK Intestines Aureobasidium pullulans medicine.anatomical_structure biology.protein Cytokines Female
Zdroj:	Scandinavian Journal of Immunology. 78:61-68
ISSN:	0300-9475
DOI:	10.1111/sji.12067
Popis:	β-Glucans, glucose polymers that are the main constituents of the outer cell walls of micro-organisms such as fungi and yeast, are known to play an immunostimulatory role. We prepared β-glucan (β-(1-3),(1-6)-D-glucan) from an edible cultured fungus through fermentation techniques using a strain of Aureobasidium pullulans ADK-34. The purity of this β-glucan preparation (AP-FBG) was demonstrated to be high through various instrumental analyses. We then examined the effects of AP-FBG on intestinal immune systems. We prepared Peyer's patch (PP) cells and measured interleukin (IL)-5, IL-6, and IgA production in culture media with AP-FBG. We found that both cytokines and IgA increased; furthermore, IL-6 secreted by PP dendritic cells (PPDCs) cultured in the presence of AP-FBG significantly increased. We tested IgA production after oral administration of AP-FBG for 2 weeks and found that AP-FBG tended to promote the production of IgA in the small intestine. Interestingly, we observed a significant increase in IgA production in the small intestines of mice treated with cyclophosphamide (CY; an immunosuppressant) after oral administration of AP-FBG diet compared with CY-treated and control diet mice. Production of IL-6 and IgA by PP cells and IL-6 production by PPDCs in AP-FBG-fed and CY-treated mice also increased. These results demonstrate that AP-FBG has the ability to activate PPDC and induce IL-6 production and IgA secretion in PP cells. These abilities were more clearly expressed when AP-FBG was orally administered in a CY-induced immunosuppressed condition. Therefore, AP-FBG may be a useful ingredient for preparing functional foods with immunomodulatory activities.
Databáze:	OpenAIRE
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