Development of an enhanced bovine viral diarrhea virus subunit vaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells
| Autor: | María José Dus Santos, Andrés Wigdorovitz, José M. Escribano, Andrea Pecora, Demian Bellido, María Sol Pérez Aguirreburualde, Darío Amilcar Malacari |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Microbiology (medical)
Diarrhea Protein subunit Guinea Pigs Vacuna a subunidad lcsh:QR1-502 Antigen-Presenting Cells Cattle Diseases Biology Microbiology Subunit vaccine Virus lcsh:Microbiology lcsh:Infectious and parasitic diseases Antigen Single chain antibody Animals lcsh:RC109-216 Baculovirus Anticuerpo de cadena simple Glycoproteins chemistry.chemical_classification Immunogenicity Glucoproteína E2 General Medicine Virology Vaccination E2 glycoprotein chemistry Immunization Vaccines Subunit biology.protein Cattle Antibody Virus de la diarrea viral bovina Glycoprotein Bovine viral diarrhea virus Single-Chain Antibodies |
| Zdroj: | Revista Argentina de Microbiología, Vol 47, Iss 1, Pp 4-8 (2015) Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA INIA: Repositorio de Resultados de Investigación del INIA |
| ISSN: | 1851-7617 |
| Popis: | Bovine viral diarrhea virus (BVDV) is an important cause of economic losses world-wide. E2 is an immunodominant protein and a promising candidate to develop subunit vaccines.To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody named APCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed inthe baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2 vaccine was the best one to evoke a humoral response, and for this reason, it was selected fora cattle vaccination experiment. All the bovines immunized with 1.5 µg of APCH-tE2 developedhigh levels of neutralizing antibodies against BVDV up to a year post-immunization, demon-strating its significant potential as a subunit vaccine. This novel vaccine is undergoing scale-upand was transferred to the private sector. Nowadays, it is being evaluated for registration asthe first Argentinean subunit vaccine for cattle. © 2014 Asociación Argentina de Microbiología. |
| Databáze: | OpenAIRE |
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