Concerted action of multiple cis-acting sequences is required for Rev dependence of late human immunodeficiency virus type 1 gene expression
| Autor: | Alexandra Bojak, Hans Wolf, Ludwig Deml, Ralf Wagner, Marcus Graf, Kurt Bieler |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
viruses
RNA Splicing Immunology Molecular Sequence Data Human immunodeficiency virus (HIV) Gene Expression Gene Products gag Replication Biology Protein Sorting Signals medicine.disease_cause Microbiology Virology Gene expression medicine Tumor Cells Cultured Humans splice RNA Messenger Nuclear export signal Gene Subgenomic mRNA Genetics Cell Nucleus Base Sequence rev Gene Products Human Immunodeficiency Virus Group-specific antigen Gene Products rev Insect Science Codon usage bias HIV-1 |
| Zdroj: | Journal of virology. 74(22) |
| ISSN: | 0022-538X |
| Popis: | Based on the human immunodeficiency virus type 1 (HIV-1) gag gene, subgenomic reporter constructs have been established allowing the contributions of different cis -acting elements to the Rev dependency of late HIV-1 gene products to be determined. Modification of intragenic regulatory elements achieved by adapting the codon usage of the complete gene to highly expressed mammalian genes resulted in constitutive nuclear export allowing high levels of Gag expression independent from the Rev/Rev-responsive element system and irrespective of the absence or presence of the isolated major splice donor. Leptomycin B inhibitor studies revealed that the RNAs derived from the codon-optimized gag gene lacking AU-rich inhibitory elements are directed to a distinct, CRM1-independent, nuclear export pathway. |
| Databáze: | OpenAIRE |
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