Influenza virus uncoating in infected cells and effect of rimantadine
| Autor: | G. V. Kornilayeva, N. K. Vorkunova, A. G. Bukrinskaya, R. A. Narmanbetova, G. K. Vorkunova |
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| Rok vydání: | 1982 |
| Předmět: |
animal structures
Rimantadine Adamantane Chick Embryo Biology medicine.disease_cause Virus Replication Virus Cell Line Viral Matrix Proteins Viral Proteins Dogs Virology Influenza A virus medicine Animals Ribonucleoprotein Cell Nucleus Viral matrix protein Virus Uncoating Viral replication Ribonucleoproteins Cytoplasm medicine.drug |
| Zdroj: | The Journal of general virology. 60(Pt 1) |
| ISSN: | 0022-1317 |
| Popis: | Uncoating of influenza virus (strain WSN) in MDCK cells was studied by following the fate of the virus labelled with radioactive precursors. The accumulation of subviral components of input virus was observed in nuclear-associated cytoplasm (NAC) obtained by treatment of the nuclei with citric acid. Two types of subviral components were found there, ribonucleoproteins (RNPs) and larger subviral particles (SVP) containing RNPs in association with M protein. SVP, with different relative amounts of M protein, were revealed in NAC, suggesting that M protein was gradually released from RNPs. The released RNPs entered the nuclei while M protein accumulated within perinuclear membranes. Thus, SVP could be regarded as probable intermediates in virus uncoating. Rimantadine prevented the release of M protein from RNPs and their penetration into the nuclei provoking the accumulation of subviral components in NAC. |
| Databáze: | OpenAIRE |
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