Development of Atropa belladonna L. Plants with High-Yield Hyoscyamine and without Its Derivatives Using the CRISPR/Cas9 System
| Autor: | Qiaozhuo Zhang, Hongping Deng, Yueyue Zheng, Jianbo Qin, Chunxue Jiang, Zhihua Liao, Youwei Zuo, Lingjiang Zeng |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Atropa belladonna L 01 natural sciences Catalysis Anisodamine lcsh:Chemistry Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound Atropa belladonna medicine Scopolamine Physical and Theoretical Chemistry CRISPR/Cas9 lcsh:QH301-705.5 Molecular Biology transgenic plant Spectroscopy Hyoscyamine Traditional medicine hyoscyamine 6β-hydroxylase Chemistry Organic Chemistry Tropane General Medicine Computer Science Applications Atropine 030104 developmental biology lcsh:Biology (General) lcsh:QD1-999 tropane alkaloids 010606 plant biology & botany medicine.drug |
| Zdroj: | International Journal of Molecular Sciences, Vol 22, Iss 1731, p 1731 (2021) |
| ISSN: | 1422-0067 |
| Popis: | Atropa belladonna L. is one of the most important herbal plants that produces hyoscyamine or atropine, and it also produces anisodamine and scopolamine. However, the in planta hyoscyamine content is very low, and it is difficult and expensive to independently separate hyoscyamine from the tropane alkaloids in A. belladonna. Therefore, it is vital to develop A. belladonna plants with high yields of hyoscyamine, and without anisodamine and scopolamine. In this study, we generated A. belladonna plants without anisodamine and scopolamine, via the CRISPR/Cas9-based disruption of hyoscyamine 6β-hydroxylase (AbH6H), for the first time. Hyoscyamine production was significantly elevated, while neither anisodamine nor scopolamine were produced, in the A. belladonna plants with homozygous mutations in AbH6H. In summary, new varieties of A. belladonna with high yields of hyoscyamine and without anisodamine and scopolamine have great potential applicability in producing hyoscyamine at a low cost. |
| Databáze: | OpenAIRE |
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