Fluorescent products and polyunsaturated fatty acids of human testes
| Autor: | W. M. Grogan, John G. Coniglio, R. Trombly, R. K. Rhamy, A. L. Tappel |
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| Rok vydání: | 1975 |
| Předmět: |
Male
Testicular Diseases Biochemistry Fluorescence Lipofuscin Lipid peroxidation chemistry.chemical_compound Column chromatography Testis Humans Silicic acid Spermatogenesis Aged chemistry.chemical_classification Chromatography Chemistry Fatty Acids Organic Chemistry Age Factors Leydig Cells Fatty acid Lipid metabolism Pigments Biological Cell Biology Middle Aged Lipid Metabolism Thin-layer chromatography Fatty Acids Unsaturated Polyunsaturated fatty acid |
| Zdroj: | Lipids. 10:591-596 |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/bf02532722 |
| Popis: | Lipid soluble fluorescent pigments from human testis were fractionated by silicic acid column chromatography and silica gel thin layer chromatography. Fluorescence analyses revealed a family of at least 3 compounds with similar fluorescence properties, including excitation and emission maxima, reversible fluorescence quenching by alkaline pH, and fluorescence quenching by heavy metal chelation. These fluorescence characteristics strongly indicated the presence of the conjugated Schiff base fluorophore-N=C-C=C-N-. The chromatographic separations employed enabled a more definitive fluorescence characterization of the lipid soluble pigments known to accumulate late in tissues with age and as a result of lipid peroxidation. Total lipids and fatty acid composition of the total lipids were determined. Polyenoic acids constituted about 40% of the total fatty acids. Histological examination of the tissues revealed some degeneration and edema, but significant spermatogenesis and normal complement of Leydig cells. |
| Databáze: | OpenAIRE |
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