Association of Wilms' tumor 1 gene single-nucleotide polymorphism rs16754 with colorectal cancer
| Autor: | Surasak Sangkhathat, Wanwisa Maneechay, Teeranut Boonpipattanapong, Samornmas Kanngern, Welawee Chaiyapan |
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| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Molecular and Clinical Oncology. 3:1401-1405 |
| ISSN: | 2049-9469 2049-9450 |
| Popis: | Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality worldwide. Our recent study demonstrated that the expression of Wilms' tumor 1 gene (WT1) is associated with surgical outcome in CRC patients. The present study aimed to investigate the genetic association of the single-nucleotide polymorphism rs16754 in the WT1 gene with the occurrence of CRC, using an age-matched case-control study design. In addition, the correlation between genotype and WT1 expression was investigated. Genomic DNA samples from 104 CRC cases, aged 15–65 years, and 208 healthy controls, were genotyped for rs16754 using the TaqMan genotyping method. The genotype distribution conformed to the Hardy-Weinberg equilibrium (P=0.80). The overall minor allele frequency (MAF) of rs16754 (allele A) was 0.33. The MAF among CRC cases was significantly higher compared with that in controls (0.39 vs. 0.31, respectively; P=0.03). The AA genotype was significantly associated with the disease (odds ratio = 2.51, 95% confidence interval: 1.24–5.07, P=0.01). Cases with the AA genotype exhibited a significantly poorer 3-year overall survival (60%), compared with those with the GG or GA genotypes (80%) (log-rank test, P |
| Databáze: | OpenAIRE |
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