Pyridine nucleotide requirements of fatty acid synthetases
| Autor: | Osamu Mitsuhashi, Konrad Bloch, Harold B. White |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Euglena gracilis
Stereochemistry Macromolecular Substances Saccharomyces cerevisiae ved/biology.organism_classification_rank.species Biology Reductase Acetates Tritium Biochemistry Models Biological Cofactor Anhydrides Mycobacterium Ligases chemistry.chemical_compound Lactones Saccharomyces Polysaccharides Animals Nucleotide Coenzyme A Molecular Biology Mycobacterium phlei chemistry.chemical_classification Triacetic acid lactone Carbon Isotopes ved/biology Fatty Acids Fatty acid Cell Biology biology.organism_classification NAD Malonates Rats Kinetics chemistry Liver biology.protein Oxidoreductases NADP Euglena |
| Zdroj: | The Journal of biological chemistry. 246(15) |
| ISSN: | 0021-9258 |
| Popis: | The multienzyme fatty acid synthetase complexes from Mycobacterium phlei, Euglena gracilis, and Saccharomyces cerevisiae require both TPNH and DPNH for optimal efficiency. In the presence of both nucleotides, the Km values for TPNH and for DPNH for all three synthetase complexes are less than 5 µm. By measuring the formation of triacetic acid lactone in the presence of either DPNH or TPNH, it has been possible to show that in M. phlei TPNH is the specific coenzyme for the β-ketoacyl reductase step while DPNH is preferentially utilized in α,β-enoyl reduction. |
| Databáze: | OpenAIRE |
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