Significant Reduction of ATP Production in PHA-Activated CD4+ Cells in 1-Day-Old Blood from Transplant Patients
| Autor: | Artur Karasyov, Washington Lim, Stanley C. Jordan, Odette A. Galera, Andy Pao, George Chaux, Elina Suviolahti, James Mirocha, Lawrence S.C. Czer, Jeffrey De Leon, Anna Petrosyan, Shili Ge, Anne Maria Jimenez, Mieko Toyoda, David Thomas |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male Daclizumab Time Factors T cell medicine.medical_treatment Antineoplastic Agents Antibodies Monoclonal Humanized Andrology Young Adult Adenosine Triphosphate Immune system Immune Tolerance medicine Humans Lymphocyte Count Phytohemagglutinins Alemtuzumab Blood Specimen Collection Transplantation biology business.industry Reproducibility of Results Immunosuppression Organ Transplantation Adenosine medicine.anatomical_structure Immunoglobulin G Monoclonal Immunology Immunologic Techniques biology.protein Female Antibody business Immunosuppressive Agents medicine.drug |
| Zdroj: | Transplantation. 94:1243-1249 |
| ISSN: | 0041-1337 |
| Popis: | Background. Global immunosuppression can be measured by assessing adenosine triphospate (ATP) levels in mitogen-stimulated CD4+ T cells.Methods. We investigated the effect of storage time on ATP levels in 234 blood samples from 18 healthy individualsand 152 transplant patients. The difference between day 0 (G13 hours post-blood draw) and day 1 (24Y37 hours) mea-surements was analyzed and compared with various factors; a subset of samples was also analyzed in 6-hour intervals.Results. The ATP levels were significantly lower on day 1 compared with that on day 0 in healthy individuals(279T159 vs 414T159 ng/mL, PG0.001) and patients (356T209 vs 455T221 ng/mL, PG0.0001). Of the 18 healthyindividuals, 17 showed ATP reduction, whereas 192 (89%) of 216 patients did so on day 1 (24.8T24.1%). In the timecourse analysis, ATP levels decreased with the blood storage time in healthy and patient samples, and the reductionbegan as early as 7 hours post-blood draw. The reduction rate was significantly higher in patient samples with low day0 ATP levels compared with samples with moderate or high levels (44.7T31.3% vs 23.2T23.6% or 18.7T15.7%;PG0.001). The reduction rate in patients treated with alemtuzumab induction was slightly higher than that indaclizumab-treated patients (28.8T24.6% vs 21.3T21.3%, P=0.09). CD4+ cell number did not change within 24 hourspost-blood draw, but CD4 expression decreased 2.0T2.8% (PG0.05).Conclusions. The ATP levels are significantly lower in 1-day-old blood compared with fresh blood, suggesting thatfresh blood should be used for assessing the T cell immune function to obtain the most accurate results.Keywords: T cell immune function assay, Blood storage, ATP levels, Monitoring of transplant patients.(Transplantation 2012;94: 00Y00) |
| Databáze: | OpenAIRE |
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