Stimulation of homologous recombination in plants expressing heterologous recombinases
| Autor: | Ewan Keir, Helena Oakey, Claire Halpin, Abdellah Barakate |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Gene Expression Translational-recoding Plant Science Biology 01 natural sciences Homology directed repair Recombinases 03 medical and health sciences Genome editing lcsh:Botany Tobacco Recombinase CRISPR Transgenes Homologous Recombination Gene 2A Gene Editing Transcription activator-like effector nuclease fungi Homozygote Gene targeting food and beverages Cell biology lcsh:QK1-989 Meiosis 030104 developmental biology Mutation Pollen Intrachromosomal homologous recombination CRISPR-Cas Systems Homologous recombination 010606 plant biology & botany Research Article |
| Zdroj: | BMC Plant Biology BMC Plant Biology, Vol 20, Iss 1, Pp 1-10 (2020) |
| ISSN: | 1471-2229 |
| Popis: | Background Current excitement about the opportunities for gene editing in plants have been prompted by advances in CRISPR/Cas and TALEN technologies. CRISPR/Cas is widely used to knock-out or modify genes by inducing targeted double-strand breaks (DSBs) which are repaired predominantly by error-prone non-homologous end-joining or microhomology-mediated end joining resulting in mutations that may alter or abolish gene function. Although such mutations are random, they occur at sufficient frequency to allow useful mutations to be routinely identified by screening. By contrast, gene knock-ins to replace entire genes with alternative alleles or copies with specific characterised modifications, is not yet routinely possible. Gene replacement (or gene targeting) by homology directed repair occurs at extremely low frequency in higher plants making screening for useful events unfeasible. Homology directed repair might be increased by inhibiting non-homologous end-joining and/or stimulating homologous recombination (HR). Here we pave the way to increasing gene replacement efficiency by evaluating the effect of expression of multiple heterologous recombinases on intrachromosomal homologous recombination (ICR) in Nicotiana tabacum plants. Results We expressed several bacterial and human recombinases in different combinations in a tobacco transgenic line containing a highly sensitive β-glucuronidase (GUS)-based ICR substrate. Coordinated simultaneous expression of multiple recombinases was achieved using the viral 2A translational recoding system. We found that most recombinases increased ICR dramatically in pollen, where HR will be facilitated by the programmed DSBs that occur during meiosis. DMC1 expression produced the greatest stimulation of ICR in primary transformants, with one plant showing a 1000-fold increase in ICR frequency. Evaluation of ICR in homozygous T2 plant lines revealed increases in ICR of between 2-fold and 380-fold depending on recombinase(s) expressed. By comparison, ICR was only moderately increased in vegetative tissues and constitutive expression of heterologous recombinases also reduced plant fertility. Conclusion Expression of heterologous recombinases can greatly increase the frequency of HR in plant reproductive tissues. Combining such recombinase expression with the use of CRISPR/Cas9 to induce DSBs could be a route to radically improving gene replacement efficiency in plants. |
| Databáze: | OpenAIRE |
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