Role of CXCR4 and SDF-1 in Mammary Tumor Metastasis in the Cat
| Autor: | Masami Morimatsu, Nobutaka Fujii, Bunei Syuto, Rina Uyama, Munekazu Nakaichi, Takayuki Nakagawa, Hozumi Yamamura, Shizu Hashimoto, Hirokazu Tamamura, Toshina Oonuma, Nobuo Sasaki |
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| Rok vydání: | 2003 |
| Předmět: |
Receptors
CXCR4 Chemokine Pathology medicine.medical_specialty Mammary Neoplasms Animal CXCR4 Cell Line Metastasis Chemokine receptor Cell Movement Tumor Cells Cultured medicine Animals RNA Messenger Neoplasm Metastasis Receptor DNA Primers Mammary tumor General Veterinary biology Reverse Transcriptase Polymerase Chain Reaction Chemotaxis medicine.disease Chemokine CXCL12 Recombinant Proteins Real-time polymerase chain reaction Cell culture Cats biology.protein Female Chemokines CXC |
| Zdroj: | Journal of Veterinary Medical Science. 65:1069-1073 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.65.1069 |
| Popis: | It has recently been suggested that the chemokine receptor CXCR4 and its ligand SDF-1 (CXCL12) promote metastasis of various cancers in humans. Since feline mammary tumors also metastasize to distant organs frequently, we used real-time quantitative PCR to examine the expression of feline CXCR4 (fCXCR4) in ten feline mammary tumor cell lines and seven feline mammary tumor tissues, and also the expression of feline SDF-1 (fSDF-1) in various organs. Cell lines derived from metastatic regions expressed more fCXCR4 than those derived from primary tumors. Mammary tumor tissues overexpressed more fCXCR4 than normal mammary tissues. Organs with high levels of fSDF-1 expression represent common sites of metastasis. Migration assays using the feline mammary tumor cell line NAC were also performed to test the activity of TN14003 and TC14012, antagonists of human CXCR4, to antagonize fCXCR4 expressed on NAC cells. TN14003 and TC14012 inhibited migration of NAC cells. We conclude that fCXCR4 may be a therapeutic target for feline mammary tumors. |
| Databáze: | OpenAIRE |
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