iTRAQ-based quantitative proteomic analysis reveals multiple effects of Emodin to Haemophilus parasuis
| Autor: | Cheng Lv, Fei Shi, Renyong Jia, Xiaoxia Liang, Li Li, Xu Song, Ye Tian, Sanjie Cao, Gang Ye, Yuanfeng Zou, Lixia Li, Jiankang Yu, Guizhou Yue, Wenzhi Tong, Ling Zhao, Lizi Yin, Zhongqiong Yin, Qiankun Cui, Changliang He |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Proteomics
0301 basic medicine Emodin Haemophilus Infections Swine medicine.drug_class 030106 microbiology Antibiotics Biophysics Virulence ATP-binding cassette transporter Biology Biochemistry Bacterial cell structure Cell Line Microbiology Haemophilus parasuis 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Haemophilus medicine Animals Bacterial disease Epithelial Cells biology.organism_classification Anti-Bacterial Agents 030104 developmental biology chemistry Growth inhibition |
| Zdroj: | Journal of Proteomics. 166:39-47 |
| ISSN: | 1874-3919 |
| DOI: | 10.1016/j.jprot.2017.06.020 |
| Popis: | Haemophilus parasuis , a symbiotic bacteria of upper respiratory tract of swine, is the etiological agent of Glasser's disease, which is characterized by fibrinous polyserositis. Emodin, exhibits antibacterial activity against H. parasuis , yet the action mode has not been fully understood. In present study, isobaric tag for relative and absolute quantification (iTRAQ) method was applied to analyze the global protein alteration of H. parasuis in response to 16 μg/mL Emodin. In total, 338 proteins exhibiting significant differential expressions were identified. It was speculated that, through application of bioinformatics analysis to theses differentially expressed proteins, Emodin mainly inhibited some key proteins expression of ABC transport system, carbohydrate metabolism pathway and bacterial cell division by inhibiting the ribosome synthesis, resulting in the growth inhibition of H. parasuis. Remarkably, nine virulence-associated proteins were detected differently expressed, further experiments revealed that after treatment with Emodin, H. parasuis could be inhibited to adhere to and invade into porcine kidney epithelial cells (PK-15 line) and exhibited increased sensitivity to serum complement in a concentration-dependent manner. Phagocytosis assay showed Emodin also could enhance phagocytic activity of porcine alveolar macrophages PAM to H. parasuis. These results indicated that Emodin also can attenuate virulence of H. parasuis and reduce infection. Biological significance The Glasser's disease caused by H. parasuis has become a typical bacterial disease and cause serious economic loss to the swine industry around the world. Antibiotics are extensively used to control the infection, but increasing antibiotic resistance has been a severe problem. Hence, novel treatment agents are needed. So far, few antibacterial agents were reported that could control H. parasuis infection. In the present study, the state-of-the-art quantitative proteomic technology was applied to uncover underlying action mechanism of Emodin. This study extends understanding of antibacterial effect of Emodin to H. parasuis at molecular level and provides useful information for further investigations. Moreover, our results provide theoretical foundation for the practical application of Emodin. |
| Databáze: | OpenAIRE |
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