Molecular detection of metastatic pancreatic carcinoma cells using a multimarker reverse transcriptase-polymerase chain reaction assay
| Autor: | Mark C. Kelley, Morito Monden, Yoshiyuki Fujiwara, Anton J. Bilchik, Christine Kuo, Dave S.B. Hoon, Shogi Nakamori, Miki Miyashiro |
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| Rok vydání: | 2000 |
| Předmět: |
Cancer Research
Pancreatic disease CA-19-9 Antigen Sensitivity and Specificity Metastasis Antigens Neoplasm Pancreatic tumor Biomarkers Tumor Tumor Cells Cultured medicine Carcinoma Humans Chorionic Gonadotropin beta Subunit Human RNA Messenger Reverse Transcriptase Polymerase Chain Reaction business.industry Cancer Proto-Oncogene Proteins c-met medicine.disease Neoplasm Proteins Pancreatic Neoplasms Blotting Southern medicine.anatomical_structure Oncology Tumor progression Cancer research N-Acetylgalactosaminyltransferases CA19-9 Pancreas business Melanoma-Specific Antigens |
| Zdroj: | Cancer. 88:1037-1044 |
| ISSN: | 1097-0142 0008-543X |
| DOI: | 10.1002/(sici)1097-0142(20000301)88:5<1037::aid-cncr13>3.0.co;2-h |
| Popis: | BACKGROUND The diagnosis of pancreatic carcinoma is often associated with a poor prognosis, because most patients already have advanced disease. A highly sensitive assay to detect the progression of pancreatic carcinoma would be of significant clinical utility. The authors developed multiple tumor mRNA markers for reverse transcriptase–polymerase chain reaction (RT-PCR) to detect metastatic tumor cells in the blood and tissue of patients with American Joint Committee on Cancer (AJCC) Stage II/III or IV pancreatic carcinoma. METHODS An RT-PCR plus Southern blot assay was used to detect mRNA of tumor markers in blood and tissues. mRNA expression of the tumor progression markers MET (hepatocyte growth factor receptor gene c-met), GalNAc-T (β1,4- N-acetyl-galactosaminyl-transferase), and β-hCG (β-human chorionic gonadotropin) was evaluated in 9 pancreatic carcinoma cell lines, 13 tumor biopsy specimens, 5 nonmalignant pancreatic tissue specimens, and blood from 33 pancreatic carcinoma patients and 32 healthy donors. RESULTS The detection limit of the assay was 1 ρg, 10 ρg, and 10 ρg for MET, GalNAc-T, and β-hCG mRNA expression, respectively. The pancreatic carcinoma cell lines expressed all three mRNA markers. Of blood specimens from 17 patients with AJCC Stage IV pancreatic carcinoma, 82%, 65%, and 76% were MET, GalNAc-T, and β-hCG mRNA positive, respectively. Of blood specimens from 16 patients with AJCC Stage II/III disease, 88% were positive for at least 1 mRNA marker. CONCLUSIONS A multiple molecular marker assay was developed to detect cancer cells in blood and tissue from patients with different stages of pancreatic carcinoma. The detection of cancer cells in the blood may be used as a marker of pancreatic tumor progression and may be useful in monitoring response to therapy. Cancer 2000;88:1037–44. © 2000 American Cancer Society. |
| Databáze: | OpenAIRE |
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