Restoration of the G1 Checkpoint and the Apoptotic Pathway Mediated by Wild-type p53 Sensitizes Squamous Cell Carcinoma of the Head and Neck to Radiotherapy
| Autor: | Kathleen F. Pirollo, Gloria L. Murphy, Yawen Chiang, Howard H. Sussman, Esther H. Chang, Zhengmei Hao, Yng-Ju Jang, Willard E. Fee, Antonina Rait, Patricia Ryan |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Programmed cell death
Pathology medicine.medical_specialty Cell cycle checkpoint Tumor suppressor gene medicine.medical_treatment Genetic enhancement Transplantation Heterologous Mice Nude Apoptosis Radiation Tolerance Mice Tumor Cells Cultured medicine Adjuvant therapy Animals Humans business.industry G1 Phase Genetic Therapy General Medicine Flow Cytometry Combined Modality Therapy Radiation therapy stomatognathic diseases Otorhinolaryngology Epidermoid carcinoma Head and Neck Neoplasms Mutation Carcinoma Squamous Cell Cancer research Female Surgery Tumor Suppressor Protein p53 business Neoplasm Transplantation |
| Zdroj: | Archives of Otolaryngology - Head and Neck Surgery. 123:507-512 |
| ISSN: | 0886-4470 |
| Popis: | Background: A significant number of squamous cell carcinomas of the head and neck (SCCHN) resist radiation treatment, the most common form of adjuvant therapy for this disease. The presence of a mutant form of the tumor suppressor gene p53 has been correlated with disruption of programmed cell death (apoptosis) and reduced cell cycle arrest, resulting in increased radiation resistance and survival. Methods and Results: We introduced by means of an adenoviral vector a functional p53 gene into a radiation-resistant SCCHN cell line that harbors mutant p53 . Replacement of wild-type p53 restored the G 1 block and apoptosis in these cells in vitro. Moreover, introduction of wild-type p53 sensitized SCCHN-induced mouse xenografts to radiotherapy in vivo. Conclusion: The combination of p53 replacement gene therapy with conventional radiotherapy may treat SCCHN more effectively. Arch Otolaryngol Head Neck Surg. 1997;123:507-512 |
| Databáze: | OpenAIRE |
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