Temporal Gene Expression Signature of Plasma Extracellular Vesicles-MicroRNAs from Post-Smolt Coho Salmon Challenged with Piscirickettsia salmonis
| Autor: | Francisco Leiva, Richard Vidal, Scarlet Bravo, Killen Ko Garcia, Osiel Guzman, Javier Moya |
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| Rok vydání: | 2021 |
| Předmět: |
Genetics
Inflammation Piscirickettsia Bacterial disease Biology Oncorhynchus kisutch Applied Microbiology and Biotechnology Extracellular Vesicles Fish Diseases MicroRNAs Immune system Gene Expression Regulation Immunity Piscirickettsiaceae Infections Gene expression medicine Piscirickettsia salmonis Animals KEGG medicine.symptom Illumina dye sequencing |
| Zdroj: | Marine biotechnology (New York, N.Y.)References. 23(4) |
| ISSN: | 1436-2236 |
| Popis: | Piscirickettsiosis is the most important bacterial disease in the Chilean salmon industry, which has borne major economic losses due to failure to control it. Cells use extracellular vesicles (EVs) as an inter-cellular communicators to deliver several factors (e.g., microRNAs) that may regulate the responses of other cells. However, there is limited knowledge about the identification and characterization of EV-miRNAs in salmonids or the effect of infections on these. In this study, Illumina sequencing technology was used to identify Coho salmon plasma EV-miRNAs upon Piscirickettsia salmonis infection at four different time points. A total of 118 novels and 188 known EV-miRNAs, including key immune teleost miRNAs families (e.g., miR-146, miR-122), were identified. A total of 245 EV-miRNAs were detected as differentially expressed (FDR |
| Databáze: | OpenAIRE |
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