RAP-1a is the main rhoptry-associated-protein-1 (RAP-1) recognized during infection with Babesia sp. BQ1 (Lintan) (B. motasi-like phylogenetic group), a pathogen of sheep in China
| Autor: | Hélène Rogniaux, Laurence Malandrin, Guiquan Guan, Emmanuelle Moreau, Claire Bonsergent, Qingli Niu |
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| Přispěvatelé: | Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR), Institut National de la Recherche Agronomique (INRA), Lanzhou Veterinary Research Institute, CAAS, Partenaires INRAE, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), 'Region Pays de la Loire' (France), INRA, European Project: 245145,EC:FP7:KBBE,FP7-KBBE-2009-3,PIROVAC(2010), Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
China [SDV]Life Sciences [q-bio] Protozoan Proteins Antibodies Protozoan Babesia Sheep Diseases Locus (genetics) Enzyme-Linked Immunosorbent Assay 03 medical and health sciences Babesiosis [SDV.IDA]Life Sciences [q-bio]/Food engineering Parasite hosting Animals [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering Pathogen Gene Antiserum Sheep General Veterinary biology Rhoptry Immune escape Gene Expression Profiling fungi Genetic Variation General Medicine biology.organism_classification Virology Recombinant Proteins body regions 030104 developmental biology Gene Expression Regulation Polyclonal antibodies biology.protein Protein expression Parasitology sense organs rap-1 |
| Zdroj: | Veterinary Parasitology Veterinary Parasitology, Elsevier, 2016, 232, pp.48-57. ⟨10.1016/j.vetpar.2016.11.013⟩ Veterinary Parasitology, 2016, 232, pp.48-57. ⟨10.1016/j.vetpar.2016.11.013⟩ |
| ISSN: | 1873-2550 0304-4017 |
| Popis: | International audience; Babesia sp. BQ1 (Lintan) is one of the parasites isolated from infected sheep in China that belongs to the B. motasi-like phylogenetic group. The rhoptry-associated-protein 1 (rap-1) locus in this group consists of a complex organization of 12 genes of three main types: 6 rap-1a variants intercalated with 5 identical copies of rap-1b and a single 3′ ending rap-1c gene. In the present study, transcription analysis performed by standard RT-PCR demonstrated that the three different rap-1 gene types and the four rap-1a variants were transcribed by the parasite cultivated in vitro. Peptides, specific for each rap-1 type gene, were selected in putative linear B-epitopes and used to raise polyclonal rabbit antisera. Using these sera, the same expression pattern of RAP-1 proteins was found in parasites cultivated in vitro or collected from acute infection whereas only RAP-1a67 was detectable in merozoite extracts. However, ELISA performed with recombinant RAP-1a67, RAP-1b or RAP-1c and sera from infected sheep demonstrated that RAP-1a67 is the main RAP-1 recognized during infection, even if some infected sheep also recognized RAP-1b and/or RAP-1c |
| Databáze: | OpenAIRE |
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