Encapsulation-free controlled release: Electrostatic adsorption eliminates the need for protein encapsulation in PLGA nanoparticles
| Autor: | Christopher K. McLaughlin, Malgosia M. Pakulska, Irja Elliott Donaghue, Molly S. Shoichet, Tyler N. Shendruk, Jaclyn M. Obermeyer, Anup Tuladhar |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Drug Compounding
Static Electricity Nanoparticle Nanotechnology 02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound Drug Delivery Systems Adsorption Polylactic Acid-Polyglycolic Acid Copolymer affinity release Static electricity Health and Medicine Lactic Acid Research Articles chemistry.chemical_classification Drug Carriers Multidisciplinary Chemistry Brain-Derived Neurotrophic Factor Biomolecule SciAdv r-articles Proteins PLGA Hydrogen-Ion Concentration central nervous system 021001 nanoscience & nanotechnology Controlled release 3. Good health 0104 chemical sciences Drug Liberation Delayed-Action Preparations drug delivery Drug delivery Nanoparticles hydrogel controlled release protein 0210 nano-technology Drug carrier Monte Carlo Method Polyglycolic Acid Research Article |
| Zdroj: | Pakulska, M M, Donaghue, I E, Obermeyer, J, Tuladhar, A, McLaughlin, C K, Shendruk, T & Shoichet, M S 2016, ' Encapsulation-free controlled release: Electrostatic adsorption eliminates the need for protein encapsulation in PLGA nanoparticles ', Science Advances, vol. 2, no. 5, e1600519 . https://doi.org/10.1126/sciadv.1600519 Science Advances |
| ISSN: | 2375-2548 |
| DOI: | 10.1126/sciadv.1600519 |
| Popis: | Researchers demonstrate that encapsulation is not necessary to achieve controlled, long-term protein release. Encapsulation of therapeutic molecules within polymer particles is a well-established method for achieving controlled release, yet challenges such as low loading, poor encapsulation efficiency, and loss of protein activity limit clinical translation. Despite this, the paradigm for the use of polymer particles in drug delivery has remained essentially unchanged for several decades. By taking advantage of the adsorption of protein therapeutics to poly(lactic-co-glycolic acid) (PLGA) nanoparticles, we demonstrate controlled release without encapsulation. In fact, we obtain identical, burst-free, extended-release profiles for three different protein therapeutics with and without encapsulation in PLGA nanoparticles embedded within a hydrogel. Using both positively and negatively charged proteins, we show that short-range electrostatic interactions between the proteins and the PLGA nanoparticles are the underlying mechanism for controlled release. Moreover, we demonstrate tunable release by modifying nanoparticle concentration, nanoparticle size, or environmental pH. These new insights obviate the need for encapsulation and offer promising, translatable strategies for a more effective delivery of therapeutic biomolecules. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|