Direct effects of interleukin-7 on the function of human T cells in vitro
| Autor: | M. E. Meniailo, V. A. Shmarov, Victor I. Seledtsov, N. M. Todosenko, O. B. Melashchenko, V. V. Malashchenko, N. D. Gazatova, Andrei G. Goncharov |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Male 0301 basic medicine T cell Clinical Biochemistry Immunology chemical and pharmacologic phenomena Biology Interferon-gamma 03 medical and health sciences Interleukin 21 medicine Humans Immunology and Allergy Cytotoxic T cell IL-2 receptor Antigen-presenting cell Interleukin 3 Interleukin-7 CD28 hemic and immune systems Natural killer T cell Molecular biology Interleukin-10 030104 developmental biology medicine.anatomical_structure Interleukin-2 Female Interleukin-4 Immunologic Memory |
| Zdroj: | European Cytokine Network. 27:102-107 |
| ISSN: | 1148-5493 |
| DOI: | 10.1684/ecn.2016.0385 |
| Popis: | СD3+ T lymphocytes were isolated by positive magnetic separation from the peripheral blood of healthy donors. In the absence of any additional activating stimuli, interleukin-7 (IL-7) was shown to augment the levels of T cells expressing CD25 activation marker both in СD4-positive and in CD4-negative effector memory (CD45RA-CD197-) T cell subsets, as well as in terminally differentiated (CD45RA+CD197-) Т cells, without significantly affecting the activation status of naive (CD45RA+CD197+) and central memory (CD45RA-CD197+) T cells. In addition, IL-7 noticeably enhanced the production of IL-2, interferon-γ (IFN-γ), and IL-10, but not IL-4, in T cells. The direct effects of IL-7 on T cell activation induced in vitro by MACSiBead™ particles coated with CD2, CD3, and CD28 antibodies (Abs) were also investigated. Upon cell activation, IL-7 significantly augmented the levels of CD25+ T cells in naive (CD45RA+CD197+), central memory (CD45RA-CD197+), and effector memory (CD45RA-CD197-) T-cell compartments. In addition, IL-7 facilitated activation of СD4- (but not CD4+) terminally differentiated effector (CD45RA+CD197-) Т cells. Finally, IL-7 was found to upregulate the production of IL-2, IFN-γ, IL-4, and IL-10 by activated T cells. In conclusion, we speculate that IL-7 is capable of enhancing functional T cell activity without causing significant functional inbalance between various T cell subsets. |
| Databáze: | OpenAIRE |
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