Quantification of cytochrome c oxidase and tissue oxygenation using CW-NIRS in a mouse cerebral cortex
| Autor: | Ying Wu, Mada Hashem, Jeff Dunn |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
biology Pulse (signal processing) Cyanide macromolecular substances Hypoxia (medical) Atomic and Molecular Physics and Optics Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine.anatomical_structure Tissue oxygenation chemistry Cerebral cortex Cortex (anatomy) medicine Biophysics biology.protein Cytochrome c oxidase Hemoglobin medicine.symptom 030217 neurology & neurosurgery 030304 developmental biology Biotechnology |
| Zdroj: | Biomed Opt Express |
| ISSN: | 2156-7085 |
| Popis: | We provide a protocol for measuring the absolute concentration of the oxidized and reduced state of cytochrome c oxidase (CCO) in the cerebral cortex of mice, using broadband continuous-wave NIRS. The algorithm (NIR-AQUA) allows for absolute quantification of CCO and deoxyhemoglobin. Combined with an anoxia pulse, this also allows for quantification of total hemoglobin, and tissue oxygen saturation. CCO in the cortex was 4.9 ± 0.1 μM (mean ± SD, n=6). In normoxia, 84% of CCO was oxidized. We include hypoxia and cyanide validation studies to show CCO can be quantified independently to hemoglobin. This can be applied to study oxidative metabolism in the many rodent models of neurological disease. |
| Databáze: | OpenAIRE |
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