Analysis of p53–RNA interactions in cultured human cells
| Autor: | Kasandra J.-L. Riley, L. James Maher |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Binding Sites
RNA-induced transcriptional silencing General transcription factor Biophysics RNA-Binding Proteins RNA RNA-dependent RNA polymerase Breast Neoplasms RNA-binding protein Cell Biology Biology Non-coding RNA Biochemistry Molecular biology Article Post-transcriptional modification Transcription (biology) Cell Line Tumor Humans Tumor Suppressor Protein p53 Molecular Biology Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 363:381-387 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2007.08.181 |
| Popis: | Tumor suppressor p53 is a well-characterized transcription factor that binds DNA. More enigmatic are the RNA-binding properties of p53 and their physiological relevance. We used three sensitive co-immunoprecipitation methods in an attempt to detect RNAs that tightly associate with p53 in cultured human cells. Although recombinant p53 protein binds RNA in a sequence-nonspecific mode, we do not detect specific in vivo RNA binding by p53. These results suggest that RNA binding is prevented by post-translational p53 modifications. A ribonucleoprotein (not p53) is purified by multiple IgG monoclonal antibodies (including anti-p53 antibodies) from both p53 +/+ and p53 null cells. Caution is therefore required in interpreting RNA co-immunoprecipitation experiments. Though not formally excluded, these results do not support models in which p53 binds specific RNA partners in vivo. |
| Databáze: | OpenAIRE |
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