An in silico, structural, and biological analysis of lactoferrin of different mammals
| Autor: | Jiri Adamec, Dielson da Silva Vieira, Camila Pereira Braga, Francisco Leydson F. Feitosa, Weslen Fabricio Pires Teixeira, Richard Costa Polveiro, Bhanwar Lal Puniya, Timothy A. Hackett, Thomas J. Butler, Pedro de Magalhães Padilha |
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| Přispěvatelé: | Universidade Estadual Paulista (UNESP), Universidade Federal de Viçosa (UFV), Technological University Dublin, University of Nebraska – Lincoln, Universidade Federal de Goiás (UFG) |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Models
Molecular Protein Conformation alpha-Helical In silico Sequence alignment Computational biology Biology Biochemistry Structure-Activity Relationship Species Specificity Leucine Structural Biology Capra hircus Animals Amino Acid Sequence Databases Protein Molecular Biology Phylogeny chemistry.chemical_classification Alanine Phylogenetic tree Lactoferrin Globular glycoprotein ExPASy Milk proteins General Medicine Protein Structure Tertiary Amino acid chemistry Mammalians biology.protein Lactotransferrin |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP |
| Popis: | Made available in DSpace on 2022-04-28T19:42:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-09-30 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Lactoferrin (LF) belongs to the family of transferrins having multifunctional roles associated with the immune system of animals. To follow the aims for this study was selected 20 sequences of LF from mammalian species to evaluate the chemical, biological, and structural properties. Bioinformatics approaches used programs such as MAFFT for sequence alignment; PartitionFinder and MrBayes for phylogenetic approaches; I-TASSER, PROCHECK, Molecular Operating Environment (MOE), SWISS Model server, Peptide DB and Expasy ProtParam to estimate the physicochemical properties, to model the protein and predicted secondary structures. A phylogenic analysis shows species with genetic similarities clustered by complexity and unique grouping between Capra hircus, Macaca mulatta, and Myotis lucifugus, since they presented more amino acids but not overall changes in the iron-binding sites or biological aspects. Structural deviations in these clusters obtained in LF from those species were found in residues 46 (position 406-450), that is part of alpha-helix, and 37 (position 295-331), that is part of the beta-sheets. Our predicted model can be used to investigate more about structural aspects of LF and be applied for medicinal research. São Paulo State University “Júlio de Mesquita Filho” (UNESP) School of Veterinary Medicine Federal University of Viçosa (UFV) Veterinary Department Environmental Sustainability and Health Institute (ESHI) School of Biological and Health Sciences Technological University Dublin Department of Biochemistry University of Nebraska – Lincoln Federal University of Goiás (UFG) Department of Veterinary Medicine São Paulo State University “Júlio de Mesquita Filho” (UNESP) Biosciences Institute São Paulo State University “Júlio de Mesquita Filho” (UNESP) School of Veterinary Medicine São Paulo State University “Júlio de Mesquita Filho” (UNESP) Biosciences Institute FAPESP: 2017/22041-1 FAPESP: 2019/02781-6 |
| Databáze: | OpenAIRE |
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