Degradation pathways of cyclic alkanes in Rhodococcus sp. NDKK48
| Autor: | Y. Fujii, Seon-Yong Chung, Motoki Kubo, Fumihiko Hasumi, Y. Sakashita, Kenzo Kubota, Daisuke Koma |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Muconic acid
Cyclic compound Time Factors Molecular Structure biology Stereochemistry General Medicine Cyclohexanecarboxylic acid biology.organism_classification Applied Microbiology and Biotechnology Gas Chromatography-Mass Spectrometry chemistry.chemical_compound Metabolic pathway Biodegradation Environmental chemistry Cyclohexanes Rhodococcus Organic chemistry Methylcyclohexane Energy source Biotechnology Benzoic acid |
| Zdroj: | Applied Microbiology and Biotechnology. 66:92-99 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-004-1623-5 |
| Popis: | The degradation pathways for cyclic alkanes (c-alkanes) in Rhodococcus sp. NDKK48 were investigated. Strain NDKK48 used dodecylcyclohexane as a sole carbon and energy source, and five metabolites in the dodecylcyclohexane degradation pathway were detected by gas-chromatography/mass spectra. The metabolites were identified as cyclohexanecarboxylic acid, cyclohexylacetic acid, 1-cyclohexene-1-acetic acid, 4-dodecylcyclohexanol, and 4-dodecylcyclohexanone. The strain degrades dodecylcyclohexane via a ring oxidation pathway and an alkyl side chain oxidation pathway. Cyclohexanecarboxylic acid was further oxidized to muconic acid via 1-cyclohexene-1-carboxylic acid and benzoic acid, and the muconic acid was finally used by strain NDKK48 for growth. Methylcyclohexane and cyclohexane were co-oxidized with hexadecane by strain NDKK48. Methylcyclohexane was degraded via a ring oxidation pathway, and the degradation pathway contained part of the Baeyer-Villiger oxidation for ring cleavage. Cyclohexane was also degraded by the same pathway as methylcyclohexane. Thus, strain NDKK48 has two pathways for the complete degradation of c-alkanes. |
| Databáze: | OpenAIRE |
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