Influence of hydrophilic amino acids and GC-content on expression of recombinant proteins used in vaccines against foot-and-mouth disease virus in Escherichia coli
| Autor: | Xiuli Wu, Yongsheng Zhang, Li Hua, Wenzhen Diao, Xiaoping Hu, Lei Zhou, Peiyin Zhang, Yongli Yu, Yue Xiao, Hua Wang, Min Wan, Liying Wang, Mingli Fang, Ming Yang, Hongfei Wei, Chengfeng Jiao |
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| Rok vydání: | 2013 |
| Předmět: |
Gene Expression
Bioengineering Biology Protein Engineering medicine.disease_cause Applied Microbiology and Biotechnology Epitope law.invention law Escherichia coli medicine Isoelectric Point Amino Acids Gene chemistry.chemical_classification Base Composition Vaccines Synthetic Linear epitope Viral Vaccines General Medicine Protein engineering Molecular biology Recombinant Proteins Amino acid Isoelectric point Biochemistry chemistry Foot-and-Mouth Disease Virus Recombinant DNA Biotechnology |
| Zdroj: | Biotechnology Letters. 36:723-729 |
| ISSN: | 1573-6776 0141-5492 |
| Popis: | Epitope-based protein expression in Escherichia coli can be improved by adjusting its amino acid composition and encoding genes. To that end, we analyzed 24 recombinant epitope proteins (rEPs) that carry multiple epitopes derived from VP1 protein of foot-and-mouth disease virus. High level expression of the rEPs was attributed to a high content of Arg, Asn, Asp and Thr, a low content of Gln, Pro and Lys, a high content of hydrophilic amino acids and a higher isoelectric point value resulting from abundant Arg. It is also attributed to the appropriate guanine and cytosine content in the encoding genes. The data provide a reference for adjusting the amino acid composition in designing epitope-based proteins used in vaccines and for adjusting the synonymous codons to improve their expressions in E. coli. |
| Databáze: | OpenAIRE |
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