HIV coating gp 120 glycoprotein-dependent prostaglandin E2 release by human cultured astrocytoma cells is regulated by nitric oxide formation
| Autor: | G.M. Lauro, Paola Rodinò, Marco Colasanti, G. Nisticò, Vincenzo Mollace |
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| Přispěvatelé: | Mollace, V, Colasanti, Marco, Rodino, P, Lauro, Gm, Nistico, G. |
| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Nitroprusside
Indomethacin Biophysics Human immunodeficiency virus (HIV) Biology Astrocytoma HIV Envelope Protein gp120 medicine.disease_cause Arginine Nitric Oxide Biochemistry Dinoprostone Cell Line chemistry.chemical_compound medicine Tumor Cells Cultured Humans Prostaglandin E2 Nitrite Molecular Biology Nitrites BLEU chemistry.chemical_classification Dose-Response Relationship Drug Biological activity Cell Biology medicine.disease Molecular biology Kinetics NG-Nitroarginine Methyl Ester chemistry Arachidonic acid Amino Acid Oxidoreductases Nitric Oxide Synthase Glycoprotein medicine.drug |
| Popis: | The role of the L-arginine-NO pathway on the formation of PGE 2 by cultured astroglial cells incubated with the HIV coating glycoprotein gp120 was investigated. Preincubation of human cultured T 67 astrocytoma cells with gp 120 (100-500 nM) produced a significant increase of nitrite (the breakdown product of NO) and PGE 2 in cell supernatants. The effect of gp 120 on both nitrite and PGE 2 production was antagonized by inhibition of NO synthase by L-NAME (20-300 μM). The inhibition of gp120-induced PGE 2 production by L-NAME was reverted by addition of arachidonic acid (30 μM), an effect antagonized by the cyclo-oxygenase inhibitor indomethacin (10 μM). Methylen bleu, an inhibitor of the biological activity of NO acting at the guanylate cyclase level failed to affect gp 120-mediated PGE 2 , release showing that the increase of cGMP subsequent to NO production was not involved in the modulatory activity of NO on arachidonic acid cascade. On the basis of present experiments we conclude that gp120-induced release of PGE 2 by astroglial cells is driven by NO, thereby contributing in the involvement of glial cells in HIV-related cerebral disorders. |
| Databáze: | OpenAIRE |
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