Expansion and characterization of adipose tissue-derived stromal cells cultured with low serum medium
| Autor: | Yoshiko Amano, Masayuki Ishihara, Bonpei Takase, Satoko Kishimoto, Hidemi Hattori, Shingo Nakamura, Yashiro Nogami, Koichi Fukuda, Yasuhiro Kanatani, Tomohiro Tanaka |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
Cell type Stromal cell Cell Transplantation Cellular differentiation Population Cell Culture Techniques Biomedical Engineering Adipose tissue Biology Collagen Type I Culture Media Serum-Free Biomaterials Mice Internal medicine medicine Animals education Cell Proliferation education.field_of_study Heparin Cell Differentiation Cell biology Transplantation Endocrinology Adipose Tissue Cell culture Fibroblast Growth Factor 2 Stromal Cells Fetal bovine serum |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. :229-236 |
| ISSN: | 1552-4981 1552-4973 |
| DOI: | 10.1002/jbm.b.31101 |
| Popis: | Adipose tissue contains a population of cells that have extensive self-renewal capacity and the ability to differentiate along multiple lineages. In addition, adipose tissue-derived stromal cells (ATSCs) are able to differentiate into various cell types that may be useful for autologous cell transplantation for defects of bone, cartilage, adipose, and tendon, etc. Most protocols for in vitro cultures of ATSCs include fetal bovine serum (FBS) as a nutritional supplement. However, in some cell cultures, it involves multiple doses of FBS, which raises a concern over possible infections as well as immunological reactions that are caused by medium-derived FBS proteins, sialic acid, etc. In this study, we were able to expand mouse ATSCs using low mouse serum media containing collagen type I, heparin-carrying polystyrene, and fibroblast growth factor (FGF)-2. These expanded mouse ATSCs maintained their multilineage potential for differentiation into adipocytes, osteoblasts, and chondrocytes. Therefore, this method, which uses autologous cells and low serum media, may be able to be utilized for clinical cell therapies. |
| Databáze: | OpenAIRE |
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