mcl1 + , the Schizosaccharomyces pombe Homologue of CTF4 , Is Important for Chromosome Replication, Cohesion, and Segregation
| Autor: | J. Richard McIntosh, Dewight R. Williams |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA Replication
Saccharomyces cerevisiae Proteins Chromosomal Proteins Non-Histone DNA damage Molecular Sequence Data Eukaryotic DNA replication Chromatids Microbiology Article Chromosome segregation Control of chromosome duplication Chromosome Segregation Gene Expression Regulation Fungal Schizosaccharomyces Sister chromatids Amino Acid Sequence Molecular Biology Genetics Sequence Homology Amino Acid biology DNA replication Proteins General Medicine biology.organism_classification DNA-Binding Proteins Schizosaccharomyces pombe Schizosaccharomyces pombe Proteins Chromosomes Fungal |
| Zdroj: | Eukaryotic Cell. 1:758-773 |
| ISSN: | 1535-9786 1535-9778 |
| DOI: | 10.1128/ec.1.5.758-773.2002 |
| Popis: | The fission yeast minichromosome loss mutant mcl1-1 was identified in a screen for mutants defective in chromosome segregation. Missegregation of the chromosomes in mcl1-1 mutant cells results from decreased centromeric cohesion between sister chromatids. mcl1 + encodes a β-transducin-like protein with similarity to a family of eukaryotic proteins that includes Ctf4p from Saccharomyces cerevisiae , sepB from Aspergillus nidulans , and AND-1 from humans. The previously identified fungal members of this protein family also have chromosome segregation defects, but they primarily affect DNA metabolism. Chromosomes from mcl1-1 cells were heterogeneous in size or structure on pulsed-field electrophoresis gels and had elongated heterogeneous telomeres. mcl1-1 was lethal in combination with the DNA checkpoint mutations rad3Δ and rad26Δ , demonstrating that loss of Mcl1p function leads to DNA damage. mcl1-1 showed an acute sensitivity to DNA damage that affects S-phase progression. It interacts genetically with replication components and causes an S-phase delay when overexpressed. We propose that Mcl1p, like Ctf4p, has a role in regulating DNA replication complexes. |
| Databáze: | OpenAIRE |
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