iSLIM: a comprehensive approach to mapping and characterizing gene regulatory networks
| Autor: | Sylvie Rockel, Sebastian J. Maerkl, Korneel Hens, Bart Deplancke, Marcel Geertz |
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| Přispěvatelé: | University of Zurich |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
SX20 Research
Technology and Development Projects Systems biology Gene regulatory network Computational biology Biology SX00 SystemsX.ch Consensus Sequence Genetics Animals Position-Specific Scoring Matrices Gene Regulatory Networks Regulatory Elements Transcriptional Nucleotide Motifs SX05 DynamiX Transcription factor Gene Gene Library Base Sequence DNA Microfluidic Analytical Techniques Drosophila melanogaster Methods Online 570 Life sciences biology Identification (biology) Transcription Factors |
| Zdroj: | Nucleic Acids Research |
| Popis: | Mapping gene regulatory networks is a significant challenge in systems biology, yet only a few methods are currently capable of systems-level identification of transcription factors (TFs) that bind a specific regulatory element. We developed a microfluidic method for integrated systems-level interaction mapping of TF-DNA interactions, generating and interrogating an array of 423 full-length Drosophila TFs. With integrated systems-level interaction mapping, it is now possible to rapidly and quantitatively map gene regulatory networks of higher eukaryotes. |
| Databáze: | OpenAIRE |
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