Clinical implication and prognostic significance of FLT3-ITD and ASXL1 mutations in Egyptian AML patients: A single-center study

Autor:	Ahmad Embaby, Reham Sameh, Samia Hussein, Sherin Elshorbagy, Norhan A. Sabbah, Haitham Mohamed, Tarek Khamis, Huda F. Ebian
Rok vydání:	2021
Předmět:	Oncology Adult Male Cancer Research medicine.medical_specialty Multivariate analysis medicine.disease_cause Single Center law.invention Cohort Studies Immunophenotyping law Risk Factors hemic and lymphatic diseases Internal medicine Genetics medicine Humans Prospective Studies Gene Polymerase chain reaction Aged Mutation business.industry Myeloid leukemia General Medicine Middle Aged Prognosis Survival Analysis Repressor Proteins Leukemia Myeloid Acute fms-Like Tyrosine Kinase 3 ASXL1 Gene Mutation Female business
Zdroj:	Cancer biomarkers : section A of Disease markers. 32(3)
ISSN:	1875-8592
Popis:	BACKGROUND: Both Fms-like tyrosine kinase 3-internal tandem duplication (FLT3-ITD) and Additional Sex Comb-like 1 (ASXL1) mutations are frequent and early genetic alteration events in acute myeloid leukemia (AML) patients. These genetic alterations may be associated with an unfavorable prognosis. OBJECTIVE: Up to our knowledge, this is the first study performed to evaluate the clinical implication and prognostic significance of FLT3-ITD and ASXL1 mutations and their coexistence on the outcome of Egyptian AML patients. METHODS: Our study included 83 patients with AML who were subjected to immunophenotyping and detection of FLT3-ITD and ASXL1 gene mutation by polymerase chain reaction (PCR) and real-time PCR, respectively. RESULTS: FLT3-ITD and ASXL1 mutations were detected in 20.5% and 18.1% of AML patients respectively. Seven patients (8.4%) had co-expression of both genes’ mutations. FLT3-ITD mutation was significantly higher in younger age, higher WBCs count and poor cytogenetic risk patients (P= 0.01, < 0.001 and 0.008 respectively). ASXL1 mutation was significantly higher in intermediate cytogenetic risk patients (P= 0.2). The mean period of survival and relapse-free survival (RFS) were significantly reduced in FLT3-ITD and ASXL1 mutations compared with their non-mutant types (P= 0.01 and 0.03 respectively). Both mutations were independent risk factors for overall survival (OS) and (RFS) in univariate and multivariate analysis in AML patients. CONCLUSION: FLT3-ITD and ASXL1 gene mutations or their coexistence can predict a poor prognosis in AML patients.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02799e89bdf1d5f91e3ef4dda6bdb65a https://pubmed.ncbi.nlm.nih.gov/34487021 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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