The comparative evaluation of apoptosis produced by leuprolide or orchiectomy on rat prostate tissue
| Autor: | Seyit Erkan Eyyupoglu, Pinar Tuzlali, Mustafa Bahadir Can Balci, Basri Cakiroglu, Aydin Ismet Hazar, Orhun Sinanoglu |
|---|---|
| Přispěvatelé: | Maltepe Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Necrosis Antineoplastic Agents Hormonal Urology Apoptosis lcsh:RC870-923 03 medical and health sciences symbols.namesake 0302 clinical medicine Atrophy Prostate Internal medicine medicine Animals Orchiectomy Fisher's exact test business.industry Reproducibility of Results medicine.disease lcsh:Diseases of the genitourinary system. Urology Rats Staining Disease Models Animal 030104 developmental biology medicine.anatomical_structure Endocrinology 030220 oncology & carcinogenesis symbols Immunohistochemistry medicine.symptom Leuprolide business |
| Zdroj: | Archivio Italiano di Urologia e Andrologia, Vol 87, Iss 4, Pp 265-269 (2016) |
| ISSN: | 2676-6795 |
| Popis: | WOS: 000440260900001 PubMed ID: 26766795 Introduction: Organisms are constantly in a balance meaning that while new cells are produced, some of the older ones die which takes place in 2 ways: necrosis or apoptosis. Apoptosis is the programmed cellular death triggered by intrinsic or extrinsic stimuli. In this study we have evaluated the apoptosis of prostate tissue generated by surgical or medical orchiectomy. Material and Method: In this experimental study, we used 36 adult male rats that were evaluated in 3 groups. The first group (Group 1) consisted of 12 rats that had bilateral orchiectomy; the second group (Group 2) included 12 rats that were given leuprolide acetate and the third group (Group 3) consisted of 12 control rats. Immunohistochemical staining of the prostate of all rats was performed and the presence of glandular atrophy and apoptosis were evaluated in the three groups. The statistical differences between the two groups were evaluated by the Fisher exact test. Results: Glandular atrophy was not determined in any rat of the control group, and the apoptotic staining was in the normal limits in all the control rats. In Leuprolide group, glandular atrophy was mild in 7 cases, and moderate in 3 rats. In 2 rats of the Leuprolide group, atrophy was not demonstrated. In surgical orchiectomy group, glandular atrophy was present in all cases. Atrophy was observed as cystic atrophy. Statistical analysis with the Fisher exact test revealed that glandular atrophy was statistically significantly more common in surgical orchiectomy group compared with Leuprolide group (p = 0,012). Conclusion: If the aim of treatment in androgen dependent prostatic adenocarcinoma or benign prostate hypertrophy is the construction of a robust apoptosis, bilateral orchiectomy generates a more powerful apoptosis compared with Leuprolide. Pathology Laboratory, Taksim Training and Research Hospital We thank Pathology Laboratory, Taksim Training and Research Hospital for laboratory facilities, technical assistance and financial aid. |
| Databáze: | OpenAIRE |
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