Copper deficiency increases fibulin-5 (DANCE/EVEC) but decreases cytochrome C oxidase VIb subunit expression in rat heart
| Autor: | Jack T. Saari, Gwen M. Dahlen, Huawei Zeng |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Protein subunit Down-Regulation Biochemistry Electron Transport Complex IV Rats Sprague-Dawley Inorganic Chemistry Gene expression medicine Animals Cytochrome c oxidase Extracellular Matrix Proteins biology Chemistry Myocardium Cytochrome c Cardiomyopathy Hypertrophic medicine.disease Molecular biology Recombinant Proteins Rats Up-Regulation Fibulin Blot Protein Subunits biology.protein Antibody Copper deficiency Copper |
| Zdroj: | Journal of Inorganic Biochemistry. 100:186-191 |
| ISSN: | 0162-0134 |
| Popis: | It has been well documented that dietary copper (Cu) deficiency causes a hypertrophic cardiomyopathy in rodent models. However, a possible alteration in gene expression has not been fully examined. The present study was undertaken to determine the effect of Cu deficiency on protein profiles in rat heart tissue with the combination of the isotope-coded affinity tag (ICAT) method and Western blotting analysis. Male Sprague-Dawley rats were fed diets that were either Cu-adequate (6.0 microg Cu/g diet n=6) or Cu-deficient (0.3 microg Cu/g diet n=6) for 5 week. The ICAT analysis suggested that high-salt buffer (HSB) protein profiles from heart tissue of Cu-deficient rats were different from those of Cu-adequate rats; seven major protein species differed by more than a 100% increase or a 50% decrease. With three available antibodies, our Western blotting analysis confirmed that there was an 85% increase in fibulin-5 (also known DANCE/EVEC) and a 71% decrease in cytochrome C oxidase (CCO) VIb subunit, but no change in succinate dehydrogenase complex (also known complex II) IP subunit in Cu-deficient rat heart. Collectively, these data may be useful in deciphering the molecular basis for the impairments of function related to the hypertrophic-cardiomyopathy of Cu-deficient rats. |
| Databáze: | OpenAIRE |
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